Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7 Journal Article
| Authors: | Selvakumar, A.; Mohanraj, B. K.; Eddy, R. L.; Shows, T. B.; White, P. C.; Dupont, B. |
| Article Title: | Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7 |
| Abstract: | The human B lymphocyte activation antigen B7 provides regulatory signals for T lymphocytes as a consequence of binding to its ligands CD28 and CTLA-4. The cDNA for B7 has previously been isolated and predicted to encode a type I membrane protein. The predicted polypeptide has a secretory signal peptide followed by two contiguous Ig-like domains, a hydrophobic transmembrane region and a short cytoplasmic tail. Here we report the exon-intron genomic organization of human B7 and the chromosomal location. The gene has six exons that span approximately 32 kilobases of DNA. Exon 1 is not translated and the second exon contains the initiation ATG codon and encodes a predicted signal peptide. This gene structure is characteristic for several eukaryotic genes with tissue-specific expression. The third and fourth exons correspond to two Ig-like domains whereas the fifth and sixth exons encode respectively the trans-membrane portion and the cytoplasmic tail. This close relationship between exons and functional domains is a characteristic feature of genes of the Ig superfamily. Cell surface expression of the B7 gene product has previously been mapped to human chromosome 12 by antibody reactivity with the B7-specific monoclonal antibody BB-1. We here demonstrate that the B7 gene is located to the q21-qter region of chromosome 3 by DNA blot analysis of human × rodent somatic cell hybrids. © 1992 Springer-Verlag. |
| Keywords: | exon; translation initiation; chromosome 12; gene expression; monoclonal antibody; amino acid sequence; molecular sequence data; eukaryota; signal peptide; rna translation; base sequence; rodentia; antigens, surface; gene structure; chromosomal localization; chromosome 3q; chromosomes, human, pair 3; tissue specificity; southern blotting; antigens, cd80; restriction mapping; hybrid cell; b lymphocyte antigen; b lymphocyte activation; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; genes, structural |
| Journal Title: | Immunogenetics |
| Volume: | 36 |
| Issue: | 3 |
| ISSN: | 0093-7711 |
| Publisher: | Springer |
| Date Published: | 1992-05-01 |
| Start Page: | 175 |
| End Page: | 181 |
| Language: | English |
| DOI: | 10.1007/bf00661094 |
| PUBMED: | 1377173 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 30 July 2019 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
-
66Selvakumar -
264Dupont
Related MSK Work