Genomic organization and allelic polymorphism of the human killer cell inhibitory receptor gene KIR103 Journal Article

  

Authors:	Selvakumar, A.; Steffens, U.; Palanisamy, N.; Chaganti, R. S. K.; Dupont, B.
Article Title:	Genomic organization and allelic polymorphism of the human killer cell inhibitory receptor gene KIR103
Abstract:	Killer cell inhibitory receptors (KIR) belong to the immunoglobulin superfamily of molecules and are expressed on natural killer (NK) cells. The KIRs of the p58/p50 family have two immunoglobulin domains and are ligands for HLA-Cw antigens, whereas the p70/p70Δ family has three immunoglobulin domains and comprises ligands for HLA-B antigens and possibly some HLA-A antigens. Members of a third KIR family, KIR103, have two immunoglobulin domains but have highest nucleotide sequence homology to the p70 family. The ligands for KIR103 on target cells are currently unknown. We here report the complete genomic organization of KIR103. It spans about 12 kb of DNA and consists of eight exons of which exon 1 and exon 2 encode the leader sequence. Exon 3 encodes the first immunoglobulin domain (γ1), and exon 4 encodes the main part of the second immunoglobulin domain (γ3), which also contains sequences contributed by exon 5 and exon 6. Exon 6 encodes the transmembrane domain, whereas exons 7 and 8 encode most of the cytoplasmic domain. KIR103 is polymorphic, and two alleles, 103AS and 103LP, are defined in this study. Additional full-length cDNA clones for KIR103 have been isolated and are shown to be formed by alternative mRNA splicing with exon skipping. Some of these truncated KIR103 cDNA could encode shorter transmembrane molecules, whereas others lack the transmembrane domain and are candidate genes for secreted KIR products. KIR103 is localized to the KIR genetic region on chromosome 19q13.4.
Keywords:	human cell; exon; cell line; alleles; amino acid sequence; molecular sequence data; alternative splicing; alternative rna splicing; natural killer cell; killer cells, natural; base sequence; chromosomes, human, pair 19; genome; hla a antigen; hla b antigen; hla c antigen; polymorphism, genetic; receptor gene; chromosome mapping; dna polymorphism; dna, complementary; receptors, immunologic; chromosome 19q; genomic structure; humans; human; priority journal; article; killer cell inhibitory receptor; chromosome 19q13.4; kir103
Journal Title:	Tissue Antigens
Volume:	49
Issue:	6
ISSN:	0001-2815
Publisher:	Wiley-Blackwell Publishing, Inc.
Date Published:	1997-06-01
Start Page:	564
End Page:	573
Language:	English
PUBMED:	9234477
PROVIDER:	scopus
DOI:	10.1111/j.1399-0039.1997.tb02803.x
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030958426&partnerID=40&md5=1194ac0f966e245ed24db1addb00dc71
Notes:	Article -- Export Date: 17 March 2017 -- Source: Scopus

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