Chromosomal localization and nucleoside diphosphate kinase-activity of human metastasis-suppressor genes NM23-1 and NM23-2 Journal Article
| Authors: | Backer, J. M.; Mendola, C. E.; Kovesdi, I.; Fairhurst, J. L.; O'Hara, B.; Eddy, R. L. Jr; Shows, T. B.; Mathew, S.; Murty, V. V. V. S.; Chaganti, R. S. K. |
| Article Title: | Chromosomal localization and nucleoside diphosphate kinase-activity of human metastasis-suppressor genes NM23-1 and NM23-2 |
| Abstract: | Human metastasis-suppressor genes nm23-1 (NME1) and nm23-2 (NME2) are implicated in control of the metastatic potential of malignant cells. Using somatic cell hybrid analysis and fluorescence in situ hybridization we co-localized both genes to 17q21.3. The 17q21 region carries the locus responsible for early-onset familial breast-ovarian cancer and several other genes that are involved in tumorigenesis and differentiation and undergo frequent rearrangements during neoplastic development. Thus, our mapping places the NME genes in a region that may be subjected to multiple selection pressures. NME1 and NME2 genes were expressed as soluble proteins in a T7 bacterial expression system. Both proteins are independently active nucleoside diphosphate kinases and readily form intra- and intermolecular disulfide bonds. The biochemical properties of these proteins may explain the diversity of mature eucaryotic nucleoside diphosphate kinases. |
| Keywords: | human genome; amplification; expression; identification; association; human breast-cancer; tumor-metastasis; insitu hybridization; binding-proteins; good prognosis |
| Journal Title: | Oncogene |
| Volume: | 8 |
| Issue: | 2 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1993-02-01 |
| Start Page: | 497 |
| End Page: | 502 |
| Language: | English |
| ACCESSION: | WOS:A1993KN00600030 |
| PROVIDER: | wos |
| PUBMED: | 8381224 |
| Notes: | Note -- Source: Wos |
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