Nestin(+)NG2(+) cells form a reserve stem cell population in the mouse prostate Journal Article

Authors: Hanoun, M.; Arnal-Estapé, A.; Maryanovich, M.; Zahalka, A. H.; Bergren, S. K.; Chua, C. W.; Leftin, A.; Brodin, P. N.; Shen, M. M.; Guha, C.; Frenette, P. S.
Article Title: Nestin(+)NG2(+) cells form a reserve stem cell population in the mouse prostate
Abstract: In the prostate, stem and progenitor cell regenerative capacities have been ascribed to both basal and luminal epithelial cells. Here, we show that a rare subset of mesenchymal cells in the prostate are epithelial-primed Nestin-expressing cells (EPNECs) that can generate self-renewing prostate organoids with bipotential capacity. Upon transplantation, these EPNECs can form prostate gland tissue grafts at the clonal level. Lineage-tracing analyses show that cells marked by Nestin or NG2 transgenic mice contribute to prostate epithelium during organogenesis. In the adult, modest contributions in repeated rounds of regression and regeneration are observed, whereas prostate epithelial cells derived from Nestin/NG2-marked cells are dramatically increased after severe irradiation-induced organ damage. These results indicate that Nestin/NG2 expression marks a novel radioresistant prostate stem cell that is active during development and displays reserve stem cell activity for tissue maintenance. © 2019 The Author(s)
Keywords: nestin; mesenchymal-to-epithelial transition; prostate stem cell
Journal Title: Stem Cell Reports
Volume: 12
Issue: 6
ISSN: 2213-6711
Publisher: Cell Press  
Date Published: 2019-06-11
Start Page: 1201
End Page: 1211
Language: English
DOI: 10.1016/j.stemcr.2019.04.019
PROVIDER: scopus
PMCID: PMC6565923
PUBMED: 31130357
Notes: Source: Scopus
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