Notch signaling enhances nestin expression in gliomas Journal Article
| Authors: | Shih, A. H.; Holland, E. C. |
| Article Title: | Notch signaling enhances nestin expression in gliomas |
| Abstract: | Recent findings suggest that Notch signaling is active in brain tumors and stem cells, and that stem cells or cells with progenitor characteristics contribute to brain tumor formation. These stem cells are marked by expression of several markers, including nestin, an intermediate filament protein. We have studied how the Notch signaling pathway affects nestin expression in brain tumors. We find that Notch receptors and ligands are expressed in vitro and in human samples of glioblastomas, the highest grade of malignant gliomas. In culture, Notch activity activates the nestin promoter. Activation of the Notch pathway also occurs in a glioblastoma multiforme mouse model induced by Kras, with translational regulation playing a role in Notch expression. Combined activation of Notch and Kras in wild-type nestin-expressing cells leads to their expansion within the subventricular zone and retention of proliferation and nestin expression. However, activation of Notch alone is unable to induce this cellular expansion. These data suggest that Notch may have a contributing role in the stem-like character of glioma cells. Copyright © 2006 Neoplasia Press, Inc. All rights reserved. |
| Keywords: | signal transduction; controlled study; human tissue; protein expression; nonhuman; glioma; brain neoplasms; cell proliferation; mouse; animals; mice; gene targeting; animal experiment; animal model; nerve tissue proteins; notch receptor; genetic transcription; in vivo study; subventricular zone; in vitro study; carcinogenesis; mice, transgenic; stem cell; gene expression regulation, neoplastic; molecular sequence data; glioblastoma; nucleotide sequence; receptors, notch; stem cells; base sequence; rats; up-regulation; k ras protein; unindexed sequence; nestin; intermediate filament proteins; chickens; notch; mouse model; translation regulation |
| Journal Title: | NeoPlasia |
| Volume: | 8 |
| Issue: | 12 |
| ISSN: | 1522-8002 |
| Publisher: | Elsevier Science Inc. |
| Date Published: | 2006-12-01 |
| Start Page: | 1072 |
| End Page: | 1082 |
| Language: | English |
| DOI: | 10.1593/neo.06526 |
| PUBMED: | 17217625 |
| PROVIDER: | scopus |
| PMCID: | PMC1790729 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 68" - "Export Date: 4 June 2012" - "CODEN: NEOPF" - "Source: Scopus" |
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