Styk1 expression is a hallmark of murine NK cells and other NK1.1(+) subsets but is dispensable for NK-cell development and effector functions Journal Article
| Authors: | Fauteux, S.; Faure, F.; Marotel, M.; Geary, C.; Daussy, C.; Sun, J. C.; Walzer, T. |
| Article Title: | Styk1 expression is a hallmark of murine NK cells and other NK1.1(+) subsets but is dispensable for NK-cell development and effector functions |
| Abstract: | To gain insight into the biology of NK cells, others and we previously identified the NK-cell signature, defined as the set of transcripts which expression is highly enriched in these cells compared to other immune subtypes. The transcript encoding the Serine/threonine/tyrosine kinase 1 (Styk1) is part of this signature. However, the role of Styk1 in the immune system is unknown. Here, we report the generation of a novel transgenic mouse model, in which Styk1 expression is invalidated and replaced by an EGFP reporter cassette. We demonstrated that Styk1 expression is a hallmark of NK cells and other NK1.1 expressing cells such as liver type 1 innate lymphoid cells (ILC1) and NK1.1 + γδ T cells. Styk1 expression is maintained by IL-15 in NK cells and negatively correlates with the expression of educating NK-cell receptors. Analysis of phosphorylation levels of mTOR substrates suggested that Styk1 could moderately contribute to the activity of the PI3K/Akt/mTOR pathway. However, Styk1-deficient NK cells develop normally and have normal in vitro and in vivo effector functions. Thus Styk1 expression is a hallmark of NK cells, ILC1 and NK1.1 + T cells but is dispensable for their development and immune functions. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
| Keywords: | protein kinase b; controlled study; protein expression; protein phosphorylation; unclassified drug; nonhuman; comparative study; cell proliferation; t lymphocyte; animal cell; chromosome 12; mouse; cell survival; reverse transcription polymerase chain reaction; cell maturation; in vivo study; in vitro study; protein tyrosine kinase; phosphatidylinositol 3 kinase; messenger rna; mammalian target of rapamycin; receptor tyrosine kinase; transcription; natural killer cell; natural killer cell receptor; natural killer cell receptor nkg2a; development; chromosome 6; leukocyte; interleukin 15; lymphocyte function; cytotoxicity assay; lymphoid cell; natural killer cells; cd94 antigen; innate lymphoid cells; priority journal; article; serine threonine tyrosine kinase 1 |
| Journal Title: | European Journal of Immunology |
| Volume: | 49 |
| Issue: | 5 |
| ISSN: | 0014-2980 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2019-05-01 |
| Start Page: | 677 |
| End Page: | 685 |
| Language: | English |
| DOI: | 10.1002/eji.201847721 |
| PUBMED: | 30690705 |
| PROVIDER: | scopus |
| PMCID: | PMC8351758 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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