Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation Journal Article
| Authors: | García-Silva, S.; Benito-Martín, A.; Sánchez-Redondo, S.; Hernández-Barranco, A.; Ximénez-Embún, P.; Nogués, L.; Mazariegos, M. S.; Brinkmann, K.; López, A. A.; Meyer, L.; Rodríguez, C.; García-Martín, C.; Boskovic, J.; Letón, R.; Montero, C.; Robledo, M.; Santambrogio, L.; Brady, M. S.; Szumera-Ciećkiewicz, A.; Kalinowska, I.; Skog, J.; Noerholm, M.; Muñoz, J.; Ortiz-Romero, P. L.; Ruano, Y.; Rodríguez-Peralto, J. L.; Rutkowski, P.; Peinado, H. |
| Article Title: | Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation |
| Abstract: | Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified from the lymphatic drainage also known as exudative seroma (ES) of stage III melanoma patients obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched in proteins resembling melanoma progression. In addition, we found that the BRAF V600E mutation can be detected in ES-derived extracellular vesicles and its detection correlated with patients at risk of relapse. © 2019 García-Silva et al. |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 216 |
| Issue: | 5 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2019-05-01 |
| Start Page: | 1061 |
| End Page: | 1070 |
| Language: | English |
| DOI: | 10.1084/jem.20181522 |
| PUBMED: | 30975894 |
| PROVIDER: | scopus |
| PMCID: | PMC6504207 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 3 June 2019 -- Source: Scopus |
