Large‐scale oligonucleotide typing for HLA‐DRB1/3/4 and HLA‐DQB1 is highly accurate, specific, and reliable Journal Article

  


Authors: Ng, J.; Hurley, C. K.; Baxter‐Lowe, L. A.; Chopek, M.; Coppo, P. A.; Hegland, J.; KuKuruga, D.; Monos, D.; Rosner, G.; Schmeckpeper, B.; Yang, S. Y.; Dupont, B.; Hartzman, R. J.
Article Title: Large‐scale oligonucleotide typing for HLA‐DRB1/3/4 and HLA‐DQB1 is highly accurate, specific, and reliable
Abstract: Abstract: DNA typing of HLA class I1 alleles of the DRB1/3/4 and DQBI loci using sequence‐specific oligonucleotide probes and polymerase chain reaction‐amplified DNA was used for the large‐scale typing of donors for the National Marrow Donor Program unrelated donor registry. The results of quality control analysis for the first 7 months of the project show the typing to be highly accurate, specific, and reliable. The percent of correctly classified HLA oligotypes based on 1652 DRB1 and 1652 DQBI assignments was greater than 99% for DRBI/DRB3/DRM and greater than 98% for DQBI. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 9011 donor samples tested at the same time by the laboratories. 1993 Blackwell Munksgaard
Keywords: methodology; polymerase chain reaction; accuracy; quality control; allele; gene amplification; bone marrow; gene locus; alleles; registries; tissue donors; dna; hla antigen class 2; hla dr antigen; hla-dr antigens; reliability; histocompatibility antigens class ii; hla typing; oligonucleotides; histocompatibility testing; oligonucleotide probe; hla dq antigen; oligonucleotide probes; human; priority journal; article; support, u.s. gov't, non-p.h.s.; hla-dq antigens; hudr ‐ hla‐w ‐ polymerase chain reaction ‐ sequence specific oligonucleotide typing
Journal Title: Tissue Antigens
Volume: 42
Issue: 5
ISSN: 0001-2815
Publisher: Wiley-Blackwell Publishing, Inc.  
Date Published: 1993-11-01
Start Page: 473
End Page: 479
Language: English
DOI: 10.1111/j.1399-0039.1993.tb02191.x
PUBMED: 8146858
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027770993&doi=10.1111%2fj.1399-0039.1993.tb02191.x&partnerID=40&md5=8d53d8ea9601f566082eccfa82e2e174
Notes: Article -- Export Date: 1 March 2019 -- Source: Scopus
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MSK Authors
  1. Soo Y Yang
    108 Yang
  2. Bo Dupont
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