New HLA-DR haplotypes containing the DRB6 pseudogene Journal Article
| Authors: | Salazar, M.; Granja, C. B.; Selvakumar, A.; Fresia, B.; Alosco, S.; Dupont, B.; Yunis, E. J. |
| Article Title: | New HLA-DR haplotypes containing the DRB6 pseudogene |
| Abstract: | An unexpected probe reaction pattern was observed in two samples during HLA-DR typing by PCR-Sequence Specific Oligonucleotide Probes. In order to confirm the unusual typings, samples were analyzed by PCR-Sequence Specific Primers, cloning, and nucleotide sequencing of the second exon of the HLA-DRB-genes. The confirmed DR, DQ phenotype for one sample was DRB 1(*)0701, DRB4(*)01, DRB5(*)0101, DRB6(*)0201, DQB(*)0602, DQB1*0202. The phenotype of other sample was DRB1(*)1602, DRB1(*)1302, DRB3(*)0301, DRB6(*)0101, DQB1(*)0501, DQB1(*)0502. The first sample has the novel combination of DRB1(*)0701 with DRB5(*)0101 and DRB6(*)0201. The second sample has either DRB6(*)0101 together with DRB1(*)1602 in absence of any DRB5 allele or DRB6*0101 together with DRB1(*)1302, DRB3(*)0301. We postulate that the most likely haplotype in sample No 1 is DRB1(*)0701, DRB5(*)0101, DRB1(*)0602 which could have arisen from gene conversion. The most likely haplotype in sample No 2, DRB1(*)1602, DRB6(*)0101, DQB1(*)0502 would have arisen from an homologous recombination event. |
| Keywords: | controlled study; human cell; exon; polymerase chain reaction; phenotype; gene amplification; haplotypes; haplotype; molecular cloning; gene conversion; genetic recombination; nucleotide sequence; hla dr antigen; hla-dr antigens; hla typing; pseudogene; pseudogenes; oligonucleotide probe; humans; human; priority journal; article; hla-dr-drb6 |
| Journal Title: | Tissue Antigens |
| Volume: | 48 |
| Issue: | 5 |
| ISSN: | 0001-2815 |
| Publisher: | Wiley-Blackwell Publishing, Inc. |
| Date Published: | 1996-11-01 |
| Start Page: | 575 |
| End Page: | 579 |
| Language: | English |
| PUBMED: | 8988540 |
| PROVIDER: | scopus |
| DOI: | 10.1111/j.1399-0039.1996.tb02671.x |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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264Dupont
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