Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable Journal Article
| Authors: | Hurley, C. K.; Maiers, M.; Ng, J.; Wagage, D.; Hegland, J.; Baisch, J.; Endres, R.; Fernandez Vina, M.; Heine, U.; Hsu, S.; Kamoun, M.; Mitsuishi, Y.; Monos, D.; Noreen, H.; Perlee, L.; Rodriguez-Marino, S.; Smith, A.; Stastny, P.; Trucco, M.; Yang, S. Y.; Yu, N.; Holsten, R.; Hartzman, R. J.; Setterholm, M. |
| Article Title: | Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable |
| Abstract: | DNA-based typing of HLA class I alleles of the HLA-A and HLA-B loci using sequence-specific oligonucleotide primers and/or probes has been used for the large-scale typing of individuals for the National Marrow Donor Program® unrelated donor registry. Typing was performed by 16 laboratories at a low level of resolution (e.g. A*01, B*07). The results of blinded quality control analysis for the first 12 months of the project show the typing to be highly accurate, specific and reliable. The total error rate based on 11,545 HLA-A and 11,428 HLA-B assignments was 1.1% for HLA-A and 1.9% for HLA-B. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 64,180 donor samples tested at the same time by the laboratories. |
| Keywords: | controlled study; human cell; sensitivity and specificity; polymerase chain reaction; accuracy; quality control; reproducibility of results; allele; bone marrow; blood; donor; register; registries; dna; laboratory; dna primers; nucleic acid hybridization; hla a antigen; hla b antigen; bone marrow transplantation; hla typing; genetic screening; oligonucleotide; dna determination; hla-a antigens; hla-b antigens; normal human; error; hla-b; histocompatibility testing; oligonucleotide probe; humans; human; priority journal; article; hla-a; bone marrow registry; sequence-specific oligonucleotide typing; sequence-specific primer typing |
| Journal Title: | Tissue Antigens |
| Volume: | 55 |
| Issue: | 4 |
| ISSN: | 0001-2815 |
| Publisher: | Wiley-Blackwell Publishing, Inc. |
| Date Published: | 2000-04-01 |
| Start Page: | 352 |
| End Page: | 358 |
| Language: | English |
| DOI: | 10.1034/j.1399-0039.2000.550409.x |
| PUBMED: | 10852387 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
