Preferential amplification of the paternal allele of the N–myc gene in human neuroblastomas Journal Article
| Authors: | Cheng, J. M.; Hiemstra, J. L.; Schneider, S. S.; Naumova, A.; Cheung, N. K. V.; Cohn, S. L.; Diller, L.; Sapienza, C.; Brodeur, G. M. |
| Article Title: | Preferential amplification of the paternal allele of the N–myc gene in human neuroblastomas |
| Abstract: | Genomic imprinting plays a role in influencing the parental origin of genes involved in cancer–specific rearrangements. We have analysed 22 neuroblastomas with N–myc amplification to determine the parental origin of the amplified N–myc allele and the allele that is deleted from chromosome 1p. We analysed DNA from neuroblastoma patients and their parents, using four polymorphisms for 1 p and three for the N–myc amplicon. We determined that the paternal allele of N–myc was preferentially amplified (12 out of 13 cases; P = 0.002). However, the paternal allele was lost from 1 p in six out of ten cases, consistent with a random distribution (P > 0.2). These results suggest that parental imprinting influences which N–myc allele is amplified in neuroblastomas, but it does not appear to affect the 1p allele that is deleted in the cases that we have examined. © 1993 Nature Publishing Group. |
| Keywords: | adult; clinical article; child, preschool; gene deletion; comparative study; polymerase chain reaction; animal; mice; allele; gene amplification; alleles; alkaline phosphatase; gene expression regulation, neoplastic; dna; neuroblastoma; heterozygosity; genes, myc; dna, neoplasm; models, genetic; oncogene c myb; genome imprinting; genes, tumor suppressor; adrenal gland neoplasms; chromosome 1p; tumor necrosis factor; dna polymorphism; southern blotting; agar gel electrophoresis; restriction fragment length polymorphism; polymorphism, restriction fragment length; father; human; male; female; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; dna probe |
| Journal Title: | Nature Genetics |
| Volume: | 4 |
| Issue: | 2 |
| ISSN: | 1061-4036 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1993-06-01 |
| Start Page: | 191 |
| End Page: | 194 |
| Language: | English |
| DOI: | 10.1038/ng0693-191 |
| PUBMED: | 8102299 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
