Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers Journal Article


Authors: Ballon, D.; Mahmood, U.; Jakubowski, A.; Koutcher, J. A.
Article Title: Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers
Abstract: A technique is described which reduced the magnetic susceptibility induced line broadening in NMR spectra obtained from three biological systems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leukemic myeioblasts yielded minimum linewidths of 1.3 Hz at 200 MHz (0.0065 ppm) after 10 min of automated shimming. 31 P spectra from an in vivo murine MCa mammary carcinoma yielded a well‐resolved phospharylcholine resonance without proton decoupling and with the resistive shim coil currents set to zero. 31 P spectra from a perfused suspension of RIF‐1 fibrosarcoma cells exhibited a γ‐nucleoside triphosphate resonance which was resolved into purine and pyrimidine components. Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company
Keywords: human cell; nonhuman; animal cell; mouse; animal; mice; tumor cells, cultured; fibrosarcoma; image enhancement; breast carcinoma; magnetic resonance spectroscopy; cell suspension; phosphorus nuclear magnetic resonance; spectroscopy; nuclear magnetic resonance; proton nuclear magnetic resonance; in vivo; mammary neoplasms, experimental; magnetism; phosphorylcholine; mice, inbred c3h; susceptibility; nucleoside triphosphate; immersion; cell strain hl 60; human; article; leukemia, experimental; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title: Magnetic Resonance in Medicine
Volume: 30
Issue: 6
ISSN: 0740-3194
Publisher: John Wiley & Sons  
Date Published: 1993-12-01
Start Page: 754
End Page: 758
Language: English
DOI: 10.1002/mrm.1910300615
PUBMED: 8139459
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 1 March 2019 -- Source: Scopus
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  1. Douglas J Ballon
    49 Ballon
  2. Jason A Koutcher
    278 Koutcher
  3. Umar Mahmood
    11 Mahmood