Cloning and characterization of a Lambert-Eaton myasthenic syndrome antigen Journal Article

  


Authors: Rosenfeld, M. R.; Wong, E.; Dalmau, J.; Manley, G.; Posner, J. B.; Sher, E.; Furneaux, H. M.
Article Title: Cloning and characterization of a Lambert-Eaton myasthenic syndrome antigen
Abstract: Lambert-Eaton myasthenic syndrome is a paraneoplastic neuromuscular disorder in which an immune response directed against a small-cell lung tumor crossreacts with antigens in the neuromuscular junction. To isolate and characterize the antigens, we screened a human fetal brain expression library with a high-titer serum from a patient with Lambert-Eaton myasthenic syndrome. This screening resulted in the isolation of a complementary DNA clone encoding an antigen we call myasthenic syndrome antigen B (MysB). Approximately 43% (3 of 7) of Lambert-Eaton myasthenic syndrome sera specifically recognized MysB fusion protein, whereas none of 34 control sera did. The predicted amino acid sequence of this clone shows a high degree of homology to the β subunit of calcium channel complexes. The MysB pre-messenger RNA is alternatively spliced to yield 3 forms of the protein differing in the domain between two highly conserved α-helical segments.
Keywords: human tissue; antigen expression; molecular cloning; cloning, molecular; lung small cell cancer; dna; amino acid sequence; molecular sequence data; antigens; rna, messenger; antigen detection; autoantigen; base sequence; sequence homology; eaton lambert syndrome; rna splicing; molecular probes; calcium channel; human; priority journal; article; lambert-eaton myasthenic syndrome; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title: Annals of Neurology
Volume: 33
Issue: 1
ISSN: 0364-5134
Publisher: Wiley Blackwell  
Date Published: 1993-01-01
Start Page: 113
End Page: 120
Language: English
DOI: 10.1002/ana.410330126
PUBMED: 8494331
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027475244&doi=10.1002%2fana.410330126&partnerID=40&md5=6025acd79311b9ff9275b1664fc1dd5f
Notes: Source: Scopus
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MSK Authors
  1. Henry M Furneaux
    57 Furneaux
  2. Jerome B Posner
    211 Posner
  3. Ellen Wong
    14 Wong
  4. Josep O Dalmau
    101 Dalmau
  5. Myrna R Rosenfeld
    36 Rosenfeld
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