Authors: | Hamann, J. C.; Kim, S. E.; Overholtzer, M. |
Article Title: | Methods for the study of entotic cell death |
Abstract: | Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation. © Springer Science+Business Media, LLC, part of Springer Nature 2019. |
Keywords: | time-lapse; entosis; cannibalism; engulfment; anoikis; cell-in-cell; soft agar |
Journal Title: | Methods in Molecular Biology |
Volume: | 1880 |
ISSN: | 1064-3745 |
Publisher: | Humana Press Inc |
Date Published: | 2019-01-01 |
Start Page: | 447 |
End Page: | 454 |
Language: | English |
DOI: | 10.1007/978-1-4939-8873-0_28 |
PUBMED: | 30610714 |
PROVIDER: | scopus |
DOI/URL: | |
Notes: | Book Chapter 28 in "Autophagy: Methods and Protocols" (ISBN: 978-1-4939-8872-3) -- Source: Scopus |