Analysis of nuclear and mitochondrial DNA alterations in thyroid and renal oncocytic tumors Journal Article
| Authors: | Tallini, G.; Ladanyi, M.; Rosai, J.; Jhanwar, S. C. |
| Article Title: | Analysis of nuclear and mitochondrial DNA alterations in thyroid and renal oncocytic tumors |
| Abstract: | The search for mitochondrial DNA (mtDNA) defects in oncocytic neoplasms has been the subject of several recent studies. We have performed qualitative and quantitative analysis of nuclear and mitochondrial DNAs in a series of 19 renal and 12 thyroid oncocytic tumors to identify specific alterations that might predict the clinical and biological behavior of these tumors. Allelic losses were seen in 2 of 19 renal tumors and 5 of 12 thyroid tumors. Among all loci (3p, 3q, 9q, 10q, 11p, 17p, and 17q) that showed losses, losses at 10q (one renal tumor and three thyroid tumors) were significantly higher (P < 0.05) than expected. Analysis of the COX I region previously reported to be altered in oncocytomas and the delta-loop region of mtDNA showed no detectable abnormalities in the restriction pattern in 10 renal and five thyroid tumors. PCR analysis of the commonly deleted 4.977-bp region failed to detect an increased frequency of mtDNA deletions in tumors compared to the controls. In addition, segmental amplification of the complete mtDNA from a renal oncocytoma using nine sets of primers revealed no gross alteration in mtDNA. Oncocytic tumors showed a mean 5-fold increase in mitochondrial DNA, which was significant (P < 0.001) when compared to corresponding controls. The relative increase in the cellular content of mtDNA may be associated with alterations in the normal coordinated interaction between the nuclear and mitochondrial genomes. Allelic deletions at 10q may be significant in the biology of oncocytic tumors, and their impact on the clinical behavior of these tumors warrants further investigation. © 1994 S. Karger AG, Basel. |
| Keywords: | clinical article; human tissue; histopathology; polymerase chain reaction; oncocytoma; kidney neoplasms; molecular sequence data; kidney tumor; nucleotide sequence; dna, neoplasm; base sequence; thyroid neoplasms; cell nucleus; dna primers; primer dna; dna, mitochondrial; mitochondria; thyroid tumor; mitochondrial dna; restriction fragment length polymorphism; polymorphism, restriction fragment length; restriction mapping; cell nucleus dna; dna probes; blotting, southern; thyroid adenoma; human; priority journal; article; dna probe |
| Journal Title: | Cytogenetics and Cell Genetics |
| Volume: | 66 |
| Issue: | 4 |
| ISSN: | 0301-0171 |
| Publisher: | S. Karger AG |
| Date Published: | 1994-01-01 |
| Start Page: | 253 |
| End Page: | 259 |
| Language: | English |
| DOI: | 10.1159/000133706 |
| PROVIDER: | scopus |
| PUBMED: | 7909283 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work