Single-cell mtDNA dynamics in tumors is driven by coregulation of nuclear and mitochondrial genomes Journal Article


Authors: Kim, M.; Gorelick, A. N.; Vàzquez-García, I.; Williams, M. J.; Salehi, S.; Shi, H.; Weiner, A. C.; Ceglia, N.; Funnell, T.; Park, T.; Boscenco, S.; O’Flanagan, C. H.; Jiang, H.; Grewal, D.; Tang, C.; Rusk, N.; Gammage, P. A.; McPherson, A.; Aparicio, S.; Shah, S. P.; Reznik, E.
Article Title: Single-cell mtDNA dynamics in tumors is driven by coregulation of nuclear and mitochondrial genomes
Abstract: The extent of cell-to-cell variation in tumor mitochondrial DNA (mtDNA) copy number and genotype, and the phenotypic and evolutionary consequences of such variation, are poorly characterized. Here we use amplification-free single-cell whole-genome sequencing (Direct Library Prep (DLP+)) to simultaneously assay mtDNA copy number and nuclear DNA (nuDNA) in 72,275 single cells derived from immortalized cell lines, patient-derived xenografts and primary human tumors. Cells typically contained thousands of mtDNA copies, but variation in mtDNA copy number was extensive and strongly associated with cell size. Pervasive whole-genome doubling events in nuDNA associated with stoichiometrically balanced adaptations in mtDNA copy number, implying that mtDNA-to-nuDNA ratio, rather than mtDNA copy number itself, mediated downstream phenotypes. Finally, multimodal analysis of DLP+ and single-cell RNA sequencing identified both somatic loss-of-function and germline noncoding variants in mtDNA linked to heteroplasmy-dependent changes in mtDNA copy number and mitochondrial transcription, revealing phenotypic adaptations to disrupted nuclear/mitochondrial balance. © The Author(s) 2024.
Keywords: controlled study; human cell; genetics; neoplasm; neoplasms; mouse; phenotype; animal; animals; mice; gene expression; genotype; pathology; cell line, tumor; tumor cell line; cell size; cell nucleus; gene dosage; dna, mitochondrial; mitochondria; mitochondrion; mitochondrial dna; dna copy number variations; copy number variation; genome, mitochondrial; single cell analysis; single-cell analysis; procedures; mitochondrial genome; cell nucleus dna; humans; human; article; whole genome sequencing; heteroplasmy; single cell rna seq
Journal Title: Nature Genetics
Volume: 56
Issue: 5
ISSN: 1061-4036
Publisher: Nature Publishing Group  
Date Published: 2024-05-01
Start Page: 889
End Page: 899
Language: English
DOI: 10.1038/s41588-024-01724-8
PUBMED: 38741018
PROVIDER: scopus
PMCID: PMC11096122
DOI/URL:
Notes: The MSK Cancer Center Support Grant (P30 CA008748) is acknowledged in the PDF -- Corresponding authors are MSK authors: Sohrab P. Shah and Ed Reznik -- Source: Scopus
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
  1. Eduard Reznik
    108 Reznik
  2. Sohrab Prakash Shah
    89 Shah
  3. Tyler Funnell
    11 Funnell
  4. Diljot Singh Grewal
    9 Grewal
  5. Nicholas Ceglia
    24 Ceglia
  6. Hongyu Shi
    14 Shi
  7. Minsoo Kim
    9 Kim
  8. Hui Jiang
    11 Jiang
  9. Nicole Rusk
    12 Rusk
  10. Cerise Tang
    11 Tang
  11. Tricia Park
    4 Park
  12. Sohrab Salehi
    9 Salehi
  13. Adam Weiner
    8 Weiner