Allelotype analysis of cervical carcinoma Journal Article
| Authors: | Mitra, A. B.; Murty, V. V. V. S.; Li, R. G.; Pratap, M.; Luthra, U. K.; Chaganti, R. S. K. |
| Article Title: | Allelotype analysis of cervical carcinoma |
| Abstract: | To identify the genetic events which may play a role in the development of cervical carcinoma, we performed a detailed allelotype analysis utilizing DNA from 53 primary tumors and corresponding normal cells and 57 polymorphic probes mapped to each of the chromosomal arms, excluding the short arms of the acrocentric chromosomes. Loss of heterozygosity (LOH) of >25% was observed at sites on 11 chromosomal arms, which included 1q (26%), 3p (35%), 3q (31%), 4q (46%), 5p (53%), 5q (38%), 6p (28%), 10q (28%), 11p (42%), 18p (38%), and Xq (26%). The most frequent LOH was noted on 4q (ADH3) and 5p (D5S19), suggesting that loss of candidate tumor suppressor genes on these chromosomal arms may play a role in the development of cervical carcinoma. The two sites of deletions identified on 5p and Xq represent novel candidate tumor suppressor gene sites which have so far not been reported in any other tumor type. Human papilloma virus status did not correlate with any of the sites which showed frequent LOH. Tp53 mutation analysis by single-strand conformation polymorphism analysis was performed in 17 tumors that either showed 17p deletions (7753, D17S5, or D17S28) or were human papilloma virus negative. One of the 7 human papilloma virus-negative tumors, which also showed LOH at the D17S28 locus, had a mutation in exon 5. This study represents the first comprehensive genetic analysis of this cancer and identifies several novel features of significance to genetic etiology of cervical carcinoma. © 1994, American Association for Cancer Research. All rights reserved. |
| Keywords: | adult; controlled study; aged; aged, 80 and over; middle aged; human cell; major clinical study; gene deletion; genotype; alleles; cancer genetics; tumor suppressor gene; dna; heterozygosity; uterine cervix cancer; x chromosome; uterine cervical neoplasms; dna determination; genes, p53; chromosome mapping; chromosomes, human, pair 17; papilloma virus; chromosomes, human, pair 5; chromosomes, human, pair 4; papillomaviridae; allelism; humans; human; female; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 54 |
| Issue: | 16 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1994-08-15 |
| Start Page: | 4481 |
| End Page: | 4487 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 8044799 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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