Deletion mapping identifies loss of heterozygosity at 5p15.1-15.2, 5q11 and 5q34-35 in human male germ cell tumors Journal Article
| Authors: | Murty, V. V. V. S.; Reuter, V. E.; Bosl, G. J.; Chaganti, R. S. K. |
| Article Title: | Deletion mapping identifies loss of heterozygosity at 5p15.1-15.2, 5q11 and 5q34-35 in human male germ cell tumors |
| Abstract: | Cytogenetic and loss of heterozygosity (LOH) studies of chromosome 5 in male germ cell tumors (GCTs) previously reported suggested the presence of one or more tumor suppressor genes (TSGs) on this chromosome which may play a role in the development of these tumors. In an attempt to further characterize allelic deletions on chromosome 5, we performed a detailed deletion mapping utilizing 66 normal-tumor DNAs from male GCTs assaying 24 polymorphic markers mapped to both the short and long arms. Thirty-seven (56%) tumors exhibited LOH at one or more loci. Loss of one allele at all informative loci was found in 15 of 37 (40.5%) cases suggesting monosomy of chromosome 5. The pattern of LOH in the remaining 22 (59.5%) tumors revealed regional losses identifying three common sites of deletions at 5p15.1-15.2, 5q11, and 5q34-35, respectively. The distribution of allelic deletions was found to be similar in all histologic subtypes with predominance of monosomy in teratomas. Thus, the present study revealed 2 types of chromosome 5 abnormalities in male GCTs, genetic monosomy and regional deletion, the latter identifying three novel sites of candidate TSGs. These data suggest that loss of genetic information on chromosome 5 plays an important role in male GCT development. |
| Keywords: | controlled study; human cell; gene deletion; dna replication; allele; gene locus; heterozygote; carcinogenesis; gene mapping; tumor suppressor gene; dna; heterozygosity; teratoma; loss of heterozygosity; chromosome deletion; germ cell tumor; chromosome analysis; marker gene; genetic markers; chromosome 5p; chromosome mapping; dna polymorphism; chromosome 5; chromosome 5q; chromosomes, human, pair 5; germinoma; monosomy; humans; human; male; priority journal; article; male germ cell tumor |
| Journal Title: | Oncogene |
| Volume: | 12 |
| Issue: | 12 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1996-06-20 |
| Start Page: | 2719 |
| End Page: | 2723 |
| Language: | English |
| PUBMED: | 8700532 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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