A membraneless organelle associated with the endoplasmic reticulum enables 3'UTR-mediated protein-protein interactions Journal Article


Authors: Ma, W.; Mayr, C.
Article Title: A membraneless organelle associated with the endoplasmic reticulum enables 3'UTR-mediated protein-protein interactions
Abstract: Approximately half of human genes generate mRNAs with alternative 3' untranslated regions (3'UTRs). Through 3'UTR-mediated protein-protein interactions, alternative 3'UTRs enable multi-functionality of proteins with identical amino acid sequence. While studying how information on protein features is transferred from 3'UTRs to proteins, we discovered that the broadly expressed RNA-binding protein TIS11B forms a membraneless organelle, called TIS granule, that enriches membrane protein-encoding mRNAs with multiple AU-rich elements. TIS granules form a reticular meshwork intertwined with the endoplasmic reticulum (ER). The association between TIS granules and the ER creates a subcellular compartment-the TIGER domain-with a biophysically and biochemically distinct environment from the cytoplasm. This compartment promotes 3'UTR-mediated interaction of SET with membrane proteins, thus allowing increased surface expression and functional diversity of proteins, including CD47 and PD-L1. The TIGER domain is a subcellular compartment that enables formation of specific and functionally relevant protein-protein interactions that cannot be established outside.
Keywords: activation; recruitment; cloning; domains; form; cell-free formation; phase-separation; granules
Journal Title: Cell
Volume: 175
Issue: 6
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 2018-11-29
Start Page: 1492
End Page: 1506
Language: English
ACCESSION: WOS:000451771700011
DOI: 10.1016/j.cell.2018.10.007
PROVIDER: wos
PUBMED: 30449617
Notes: Article -- Source: Wos
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MSK Authors
  1. Christine Mayr
    14 Mayr
  2. Weirui Ma
    4 Ma