Alternative 3 ' UTRs act as scaffolds to regulate membrane protein localization Journal Article

Authors: Berkovits, B. D.; Mayr, C.
Article Title: Alternative 3 ' UTRs act as scaffolds to regulate membrane protein localization
Abstract: About half of human genes use alternative cleavage and polyadenylation (ApA) to generate messenger RNA transcripts that differ in the length of their 3' untranslated regions (3' UTRs) while producing the same protein(1-3). Here we show in human cell lines that alternative 3' UTRs differentially regulate the localization of membrane proteins. The long 3' UTR of CD47 enables efficient cell surface expression of CD47 protein, whereas the short 3' UTR primarily localizes CD47 protein to the endoplasmic reticulum. CD47 protein localization occurs post-translationally and independently of RNA localization. In our model of 3' UTR-dependent protein localization, the long 3' UTR of CD47 acts as a scaffold to recruit a protein complex containing the RNA-binding protein HuR (also known as ELAVL1) and SET4 to the site of translation. This facilitates interaction of SET with the newly translated cytoplasmic domains of CD47 and results in subsequent translocation of CD47 to the plasma membrane via activated RAC1 (ref. 5). We also show that CD47 protein has different functions depending on whether it was generated by the short or long 3' UTR isoforms. Thus, ApA contributes to the functional diversity of the proteome without changing the amino acid sequence. 3' UTR-dependent protein localization has the potential to be a widespread trafficking mechanism for membrane proteins because HuR binds to thousands of mRNAs(6-9), and we show that the long 3' UTRs of CD44, ITGA1 and TNFRSF13C, which are bound by HuR, increase surface protein expression compared to their corresponding short 3' UTRs. We propose that during translation the scaffold function of 3' UTRs facilitates binding of proteins to nascent proteins to direct their transport or function-and this role of 3' UTRs can be regulated by ApA.
Keywords: rna-binding proteins; immunoprecipitation; in-vivo; cell; messenger-rna; sites; gtp-binding; set protein; hur; cd47
Journal Title: Nature
Volume: 522
Issue: 7556
ISSN: 0028-0836
Publisher: Nature Publishing Group  
Date Published: 2015-06-18
Start Page: 363
End Page: 367
Language: English
ACCESSION: WOS:000356425400058
DOI: 10.1038/nature14321
PUBMED: 25896326
PMCID: PMC4697748
Notes: Article -- Source: Wos
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MSK Authors
  1. Christine Mayr
    17 Mayr