A new polymorphic site in intron 2 of TP53 characterizes LOH in human tumors by PCR-SSCP Journal Article

  


Authors: Oliva, M. R.; Saez, G. T.; Latres, E.; Cordon-Cardo, C.
Article Title: A new polymorphic site in intron 2 of TP53 characterizes LOH in human tumors by PCR-SSCP
Abstract: Many human cancers present deletions of the short arm of chromosome 17, which includes the TP53 locus. We detected a new polymorphism in intron 2 of the TP53 gene using PCR-SSCP and used this polymorphic site as a marker to detect loss of heterozygosity in 135 human tumors (73 soft tissue sarcomas, and 48 colorectal and 14 bladder carcinomas). Heterozygosity for this site was 41.5% in this study group and tumor-specific loss of alleles occurred in 43% of informative cases. Allelic losses were more frequently detected at this site than at that in which restriction fragment length polymorphism (RFLP) is located, as detected by the pHp53B probe. It is concluded that this novel approach has several advantages, including detection of a high incidence of informative cases and minimal tissue requirements. © Lippincott-Raven Publishers.
Keywords: controlled study; human tissue; polymerase chain reaction; intron; introns; heterozygote; protein p53; colorectal carcinoma; sarcoma; colorectal neoplasms; molecular sequence data; heterozygosity; soft tissue sarcoma; base sequence; bladder neoplasms; bladder carcinoma; chromosome deletion; chromosome loss; soft tissue neoplasms; genes, p53; chromosome 17p; restriction fragment length polymorphism; polymorphism, restriction fragment length; single strand conformation polymorphism; polymorphism, single-stranded conformational; tp53 gene; human tumors; human; priority journal; article; polymorphism (genetics); support, non-u.s. gov't; support, u.s. gov't, p.h.s.; pcr-allelotyping
Journal Title: Diagnostic Molecular Pathology
Volume: 4
Issue: 1
ISSN: 1052-9551
Publisher: Lippincott Williams & Wilkins  
Date Published: 1995-03-01
Start Page: 54
End Page: 58
Language: English
DOI: 10.1097/00019606-199503000-00010
PUBMED: 7735557
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028959532&doi=10.1097%2f00019606-199503000-00010&partnerID=40&md5=095f0f6131ccfe9a80ac27d553b371a5
Notes: Article -- Export Date: 28 August 2018 -- Source: Scopus
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MSK Authors
  1. Carlos Cordon-Cardo
    612 Cordon-Cardo
  2. Esther Latres
    10 Latres
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