Molecular genetic alterations of chromosome 17 and p53 nuclear overexpression in human bladder cancer Journal Article
| Authors: | Dalbagni, G.; Presti, J. C. Jr; Reuter, V. E.; Zhang, Z. F.; Sarkis, A. S.; Fair, W. R.; Cordon-Cardo, C. |
| Article Title: | Molecular genetic alterations of chromosome 17 and p53 nuclear overexpression in human bladder cancer |
| Abstract: | We set out to define the alterations of chromosome 17 in human bladder tumors and to correlate p53 nuclear over-expression with 17p deletions in those neoplasms. We studied 60 bladder tumors by restriction fragment-length polymorphism analysis directed at five different loci on chromosome 17. The same tumors were studied with a panel of mouse monoclonal antibodies (PAbl801, PAb240, and PAbl620) to mutant and wild-type p53 proteins using immunohistochemistry. Deletion of 17p correlated with grade (p = 0.039), stage (p = 0.004), and the presence of vascular invasion (p = 0.056). None of the pathologic parameters correlated with 17q deletions. p53 nuclear overexpression correlated with grade (p = 0.027), stage (p = 0.008), vascular invasion (p = 0.021), and the presence of nodal metastases (p = 0.007). In superficial (Ta) lesions, 17p was not deleted, whereas 55% of Tl and T2-T4 tumors showed a loss of heterozygosity. Mutations of p53 as detected by immunohistochemistry were seen in superficial as well as invasive tumors, whereas loss of heterozygosity was seen only in invasive tumors. A strong correlation was found between the presence of mutation and the loss of heterozygosity of the remaining allele (p = 0.0003). Additional follow-up and further studies are required to better define the role of p53 nuclear overexpression and 17p deletions as markers of tumor progression in human bladder cancer. © 1993 Raven-press. Ltd. |
| Keywords: | immunohistochemistry; human tissue; gene mutation; mutation; cancer grading; incidence; alleles; bladder cancer; protein p53; monoclonal antibody; cancer invasion; immunoenzyme techniques; tumor suppressor gene; heterozygosity; intermethod comparison; bladder neoplasms; tumor growth; chromosome deletion; chromosome 17; p53; chromosome 17q; genes, p53; chromosome 17p; chromosomes, human, pair 17; restriction fragment length polymorphism; polymorphism, restriction fragment length; human; article; support, u.s. gov't, p.h.s.; flp |
| Journal Title: | Diagnostic Molecular Pathology |
| Volume: | 2 |
| Issue: | 1 |
| ISSN: | 1052-9551 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1993-03-01 |
| Start Page: | 4 |
| End Page: | 13 |
| Language: | English |
| PUBMED: | 7904525 |
| PROVIDER: | scopus |
| DOI: | 10.1097/00019606-199303000-00002 |
| DOI/URL: | |
| Notes: | Source: Scopus |
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