Non-redundant ISGF3 components promote NK cell survival in an auto-regulatory manner during viral infection Journal Article

Authors: Geary, C. D.; Krishna, C.; Lau, C. M.; Adams, N. M.; Gearty, S. V.; Pritykin, Y.; Thomsen, A. R.; Leslie, C. S.; Sun, J. C.
Article Title: Non-redundant ISGF3 components promote NK cell survival in an auto-regulatory manner during viral infection
Abstract: Natural killer (NK) cells are innate lymphocytes that possess adaptive features, including antigen-specific clonal expansion and long-lived memory responses. Although previous work demonstrated that type I interferon (IFN) signaling is crucial for NK cell expansion and memory cell formation following mouse cytomegalovirus (MCMV) infection, the global transcriptional mechanisms underlying type I IFN-mediated responses remained to be determined. Here, we demonstrate that among the suite of transcripts induced in activated NK cells, IFN-α is necessary and sufficient to promote expression of its downstream transcription factors STAT1, STAT2, and IRF9, via an auto-regulatory, feedforward loop. Similar to STAT1 deficiency, we show that STAT2- or IRF9-deficient NK cells are defective in their ability to expand following MCMV infection, in part because of diminished survival rather than an inability to proliferate. Thus, our findings demonstrate that individual ISGF3 components are crucial cell-autonomous and non-redundant regulators of the NK cell response to viral infection. Using RNA-seq and ChIP-seq, Geary et al. investigate the impacts of type I interferon on NK cells during MCMV infection and demonstrate crucial and non-redundant roles for STAT1, STAT2, and IRF9 in promoting cytotoxicity and survival of antiviral NK cells. © 2018 The Author(s)
Keywords: interferon; transcriptional regulation; nk cells; mcmv; viral infection; irf9; stat2
Journal Title: Cell Reports
Volume: 24
Issue: 8
ISSN: 2211-1247
Publisher: Cell Press  
Date Published: 2018-08-21
Start Page: 1949
End Page: 1957.e6
Language: English
DOI: 10.1016/j.celrep.2018.07.060
PROVIDER: scopus
PUBMED: 30134157
Notes: Article -- Export Date: 4 September 2018 -- Source: Scopus
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MSK Authors
  1. Joseph C Sun
    66 Sun
  2. Christina Leslie
    111 Leslie
  3. Clair   Geary
    9 Geary
  4. Nicholas M Adams
    10 Adams
  5. Colleen M Lau
    7 Lau
  6. Sofia Elena Vaccarino Gearty
    1 Gearty