Non-invasive differentiation of benign renal tumors from clear cell renal cell carcinomas using clinically translatable hyperpolarized (13)C pyruvate magnetic resonance Journal Article

   

Authors:	Sriram, R.; Van Criekinge, M.; DeLos Santos, J.; Keshari, K. R.; Wilson, D. M.; Peehl, D.; Kurhanewicz, J.; Wang, Z. J.
Article Title:	Non-invasive differentiation of benign renal tumors from clear cell renal cell carcinomas using clinically translatable hyperpolarized (13)C pyruvate magnetic resonance
Abstract:	Incidental detection of localized renal tumors at imaging is increasing. Conventional imaging cannot reliably differentiate the 20% of these tumors that are benign from malignant renal cell carcinomas (RCCs), leading to unnecessary surgical resection and resulting morbidity. Here, we investigated hyperpolarized C-13 pyruvate metabolism in live patient-derived renal tumor tissue slices using a novel magnetic resonance-compatible bioreactor platform. We show, for the first time, that clear cell RCCs (ccRCCs), which constitute 70%-80% of all RCCs, exhibit increased lactate production and rapid lactate efflux when compared with benign renal tumors. This difference is because of increased lactate dehydrogenase A and monocarboxylate transporter 4 expression in ccRCCs. Thus, RCCs can be differentiated from benign renal tumors by assessing this distinctive metabolic phenotype using clinically translatable hyperpolarized C-13 pyruvate magnetic resonance.
Keywords:	metabolism; renal cell carcinoma; model; bioreactor; prostate-cancer; features; masses; consequences; lactate; export; aerobic glycolysis; lactate efflux; dynamic nuclear polarization; transporter mct4; hyperpolarized c-13 magnetic resonance; patient-derived tissue slice cultures
Journal Title:	Tomography
Volume:	2
Issue:	1
ISSN:	2379-1381
Publisher:	MDPI
Date Published:	2016-03-01
Start Page:	35
End Page:	42
Language:	English
ACCESSION:	WOS:000393868500005
DOI:	10.18383/j.tom.2016.00106
PROVIDER:	wos
PMCID:	PMC4876723
PUBMED:	27227168
Notes:	Article -- Source: Wos

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