Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese hospital Journal Article
| Authors: | Huang, B.; He, Y.; Ma, X.; Cai, R.; Zeng, J.; Lu, Y.; Zhang, W.; Lan, K.; E, S.; Tang, Y. W.; Kreiswirth, B. N.; Chen, C.; Chen, L. |
| Article Title: | Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese hospital |
| Abstract: | Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations. © 2018 American Society for Microbiology. All Rights Reserved. |
| Keywords: | controlled study; promoter region; nonhuman; gene expression; in vitro study; antibiotic resistance; 5' untranslated region; nucleotide sequence; escherichia coli; plasmid; beta galactosidase; genomics; bacterium isolate; plasmids; china; bacterial gene; colistin; promoter; gene expression level; klebsiella pneumoniae; next generation sequencing; priority journal; article; mcr-1 gene |
| Journal Title: | Antimicrobial Agents and Chemotherapy |
| Volume: | 62 |
| Issue: | 5 |
| ISSN: | 0066-4804 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2018-05-01 |
| Start Page: | e00018-18 |
| Language: | English |
| DOI: | 10.1128/aac.00018-18 |
| PROVIDER: | scopus |
| PMCID: | PMC5923141 |
| PUBMED: | 29463536 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2018 -- Source: Scopus |
