Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese hospital Journal Article


Authors: Huang, B.; He, Y.; Ma, X.; Cai, R.; Zeng, J.; Lu, Y.; Zhang, W.; Lan, K.; E, S.; Tang, Y. W.; Kreiswirth, B. N.; Chen, C.; Chen, L.
Article Title: Promoter variation and gene expression of mcr-1-harboring plasmids in clinical isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese hospital
Abstract: Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations. © 2018 American Society for Microbiology. All Rights Reserved.
Keywords: controlled study; promoter region; nonhuman; gene expression; in vitro study; antibiotic resistance; 5' untranslated region; nucleotide sequence; escherichia coli; plasmid; beta galactosidase; genomics; bacterium isolate; plasmids; china; bacterial gene; colistin; promoter; gene expression level; klebsiella pneumoniae; next generation sequencing; priority journal; article; mcr-1 gene
Journal Title: Antimicrobial Agents and Chemotherapy
Volume: 62
Issue: 5
ISSN: 0066-4804
Publisher: American Society for Microbiology  
Date Published: 2018-05-01
Start Page: e00018-18
Language: English
DOI: 10.1128/aac.00018-18
PROVIDER: scopus
PMCID: PMC5923141
PUBMED: 29463536
DOI/URL:
Notes: Article -- Export Date: 1 June 2018 -- Source: Scopus
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  1. Yi-Wei Tang
    188 Tang