Cloning and characterization of rat Ku70: Involvement of Ku autoantigen in the heat-shock response Journal Article

  


Authors: Yang, S. H.; Nussenzweig, A.; Yang, W. H.; Kim, D.; Li, G. C.
Article Title: Cloning and characterization of rat Ku70: Involvement of Ku autoantigen in the heat-shock response
Abstract: To study the role of the Ku autoantigen in the heat-shock response, we have cloned the 70-kDa subunit (the DNA-binding component of Ku) from a rat cDNA library. The deduced amino acid sequence of rat Ku70 bears extensive homology with the human and murine counterpart (83 and 97% identity, respectively). Overexpression of Ku70 in rat fibroblasts results in the specific repression of hsp-70 upon heat shock. The inhibition of induction of hsp-70 was greatest in cells expressing the highest level of Ku70. Induction of other heat-shock proteins besides hsp-70 by hyperthermia appears normal, as does the activation of the heat-shock transcription factor, HSF-1. It is likely that other factors besides HSF-1 are involved in controlling heat- shock gene transcription. While the formation of the Ku70 and Ku80 complex is important for repair of DNA double-strand breaks, our data suggest that the 70-kDa subunit of Ku plays a role in regulating hsp-70 expression.
Keywords: dna-binding proteins; nonhuman; antigen expression; animal cell; animals; transcription factor; phosphorylation; animalia; transcription factors; nuclear proteins; gene expression regulation; molecular cloning; cloning, molecular; dna; amino acid sequence; molecular sequence data; rat; murinae; autoantigen; autoantigens; rats; heat shock protein 70; gene induction; sequence homology; dna helicases; heat shock; hsp70 heat-shock proteins; antigens, nuclear; priority journal; article
Journal Title: Radiation Research
Volume: 146
Issue: 6
ISSN: 0033-7587
Publisher: Radiation Research Society  
Date Published: 1996-12-01
Start Page: 603
End Page: 611
Language: English
PUBMED: 8955709
PROVIDER: scopus
DOI: 10.2307/3579375
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029849232&partnerID=40&md5=5ac7d4792aa8474e7594e103b0c7f15c
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Dooha Kim
    21 Kim
  2. Gloria C Li
    132 Li
  3. Wei Hong Yang
    19 Yang
  4. Andre Nussenzweig
    18 Nussenzweig
  5. Shao-Hua Yang
    9 Yang
Related MSK Work