Down-regulation of gadd153 by c-myc in rat fibroblasts and its effect on cell growth and radiation-induced apoptosis Journal Article
| Authors: | Chen, C.; Nussenzweig, A.; Guo, M.; Kim, D.; Li, G. C.; Ling, C. C. |
| Article Title: | Down-regulation of gadd153 by c-myc in rat fibroblasts and its effect on cell growth and radiation-induced apoptosis |
| Abstract: | Using differential display reverse transcription PCR (DDRT-PCR), we found that a 360 bp cDNA fragment was absent in several c-myc transfected rat fibroblasts: REC:myc, REC:myc+ras and rat1-myc. These cells also showed enhanced sensitivity to gamma ray-induced apoptosis. This cDNA fragment was present in the parental REC (Rat Embryo Cells) and rat1 cells, and in c-Ha-ras transfected REC (REC:ras), all of which were relatively resistant to gamma ray-induced apoptosis. The cDNA fragment was subsequently cloned and used as a probe to screen a rat1 cDNA library, and identified as one of the growth arrest and DNA damaging-inducible genes, gadd153. In addition to the down-regulation of rat gadd153 in all the c-myc transfectants, methyl methanesulfonate (MMS)-induced transcription of the gadd153 was attenuated. The rat1-myc cells, when successfully transfected with and stably expressing the rat gadd153, showed a significantly longer doubling time compared to the parental cells. However, overexpression of gadd153 in rat1-myc cells did not affect gamma ray-induced apoptosis. Thus, the suppression of gadd153 appears to be inversely correlated with that of myc, but not involved in the myc-dependent apoptotic pathway. |
| Keywords: | controlled study; dna-binding proteins; nonhuman; animal cell; animals; dna damage; gene; cell division; reverse transcription polymerase chain reaction; apoptosis; down-regulation; oncogene h ras; animalia; transcription factors; gene expression regulation; molecular cloning; cloning, molecular; molecular sequence data; rna, messenger; genes, myc; rat; growth regulation; fibroblast; fibroblasts; radiosensitivity; rats; gene induction; c-myc; oncogene c myc; complementary dna; ccaat-enhancer-binding proteins; rats, inbred f344; growth arrest; mesylic acid methyl ester; gamma radiation; dna library; transcription factor chop; dna transfection; priority journal; article; gadd153 |
| Journal Title: | Oncogene |
| Volume: | 13 |
| Issue: | 8 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1996-10-17 |
| Start Page: | 1659 |
| End Page: | 1665 |
| Language: | English |
| PUBMED: | 8895511 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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