Apoptosis in heat-induced cell killing: The protective role of hsp-70 and the sensitization effect of the c-myc gene Journal Article
| Authors: | Li, W. X.; Chen, C. H.; Ling, C. C.; Li, G. C. |
| Article Title: | Apoptosis in heat-induced cell killing: The protective role of hsp-70 and the sensitization effect of the c-myc gene |
| Abstract: | We studied heat-induced apoptosis and loss of clonogenicity in Rat-1 fibroblasts, thermotolerant Rat-1 (TT Rat-1), Rat-1 transfected with the human hsp-70 gene (M21) and Rat-1 transfected with the human c-myc proto- oncogene (Rat-1:myc). Relative to Rat-1, TT Rat-1 and M21 cells are heat- resistant, but Rat-1:myc cells are heat-sensitive, in terms of both apoptosis and clonogenic survival. The apoptotic fractions, assayed 24 h after a heat treatment of 60 min at 44°C, are about 20% for Rat-1, 7% for TT Rat-1, 10% for M21 and 70% for Rat-1:myc cells, respectively. Most of the apoptotic cells detach from substratum within 1 day of heat treatment and exhibit morphological changes, chromatin condensation and DNA fragmentation. The results of this study suggest that (1) apoptosis is an important mechanism of heat-induced cell killing in some cell lines, (2) apoptosis-mediated cell killing manifests rapidly (relative to clonogenic assay) after a heat treatment, and (3) overexpression of the human hsp-70 gene reduces, whereas expression of the human c-myc proto-oncogene enhances, heat induced apoptosis. Lastly, the effects of the hsp-70 and c-myc genes on the thermosensitivity of cells are correlated with their modulation of apoptosis. |
| Keywords: | controlled study; nonhuman; animal cell; animals; cell death; dna damage; cell survival; cell structure; apoptosis; cell line; transfection; radiation response; time factors; animalia; genetic vectors; genetic transfection; kinetics; genes, myc; recombinant proteins; rat; immunoblotting; rats; heat shock protein 70; oncogene c myc; clonogenic assay; proto-oncogene proteins c-myc; clone cells; cell killing; complementary dna; retroviridae; hsp70 heat-shock proteins; heat treatment; northern blotting; dna fragment; chromatin condensation; heat; heat sensitivity; humans; priority journal; article |
| Journal Title: | Radiation Research |
| Volume: | 145 |
| Issue: | 3 |
| ISSN: | 0033-7587 |
| Publisher: | Radiation Research Society |
| Date Published: | 1996-03-01 |
| Start Page: | 324 |
| End Page: | 330 |
| Language: | English |
| DOI: | 10.2307/3578988 |
| PUBMED: | 8927700 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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