Glucose deprivation-induced cytotoxicity in drug resistant human breast carcinoma MCF-7/ADR cells: Role of c-myc and bcl-2 in apoptotic cell death Journal Article
| Authors: | Lee, Y. J.; Galoforo, S. S.; Berns, C. M.; Tong, W. P.; Kim, H. R. C.; Corry, P. M. |
| Article Title: | Glucose deprivation-induced cytotoxicity in drug resistant human breast carcinoma MCF-7/ADR cells: Role of c-myc and bcl-2 in apoptotic cell death |
| Abstract: | We investigated the effect of glucose deprivation treatment on clonogenicity in multidrug-resistant human breast carcinoma MCF-7/ADR cells. Survival of MCF-7/ADR cells decreased exponentially up to 8 hours of incubation in the glucose-free medium. The surviving fraction of these cells for 8 hours of glucose-deprivation treatment was 1.5 x 10-3. Photomicrographs and gel electrophoresis data suggest that glucose deprivation-induced cell death is associated with apoptosis. Data from western and northern blots showed an induction of c-myc gene expression during treatment with glucose-free medium in MCF-7/ADR cells. MCF-7/ADR cells transfected with c-myc antisense oligodeoxynucleotides became resistant to glucose deprivation-induced apoptosis. Overexpression of bcl-2 gene protected MCF-7/ADR cells from this apoptotic cell death. Taken together, these results indicate that c-myc expression is a necessary component of glucose-free medium induced apoptosis and bcl-2 prevents apoptotic death induced by c-myc. |
| Keywords: | human cell; cell survival; apoptosis; cytotoxicity; drug resistance; drug resistance, neoplasm; tumor cells, cultured; breast neoplasms; gene expression regulation, neoplastic; genes, myc; glucose; c-myc; oncogene c myc; carcinoma cell; clonogenesis; drug resistance, multiple; bcl-2; etiology; antisense oligodeoxynucleotide; genes, bcl-2; humans; human; priority journal; article; glucose deprivation; mcf-7/adr |
| Journal Title: | Journal of Cell Science |
| Volume: | 110 |
| Issue: | 5 |
| ISSN: | 0021-9533 |
| Publisher: | Company of Biologists |
| Date Published: | 1997-03-01 |
| Start Page: | 681 |
| End Page: | 686 |
| Language: | English |
| PUBMED: | 9092950 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
