Genomic organization of the human p57(KIP2) gene and its analysis in the G401 Wilms' tumor assay Journal Article
| Authors: | Reid, L. H.; Crider-Miller, S. J.; West, A.; Lee, M. H.; Massagué, J.; Weissman, B. E. |
| Article Title: | Genomic organization of the human p57(KIP2) gene and its analysis in the G401 Wilms' tumor assay |
| Abstract: | The p57KIP2 gene encodes an inhibitor of cyclin-dependent kinase activity, which negatively regulates cell cycle progression. The human p57 gene is located in 11p15.5, a region of DNA frequently altered in neoplasia. We have isolated a human genomic clone and mapped the p57 gene to a 2.2-kb region between D11S648 and D11S679. Sequence analysis revealed that the coding DNA of the human p57 gene is divided by a 0.5-kb intron. A second intron was detected in the 3′ untranslated region, indicating that the human p57 gene contains at least three exons. Our previous work with somatic cell hybrids mapped a tumor suppressor gene for the G401 Wilms' tumor cell line to a ∼500-kb region of 11p15.5 that includes p57. Northern blot analysis detected a 0.8-kb p57 transcript in several of the G401 hybrid lines. However, p57 expression did not correlate with tumor suppression. These results suggest that p57 is not responsible for the tumor suppression observed in our somatic cell hybrid assay. |
| Keywords: | gene deletion; nonhuman; genetic analysis; animal cell; animals; genes; kidney neoplasms; nuclear proteins; cancer genetics; tumor suppressor gene; molecular sequence data; base sequence; genome; dna sequence; chromosomes, human, pair 11; dna determination; nephroblastoma; chromosome mapping; dna, complementary; cricetinae; wilms tumor; hybrid cell; hybrid cells; genes, wilms tumor; cyclin-dependent kinase inhibitor p57; humans; human; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 56 |
| Issue: | 6 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1996-03-15 |
| Start Page: | 1214 |
| End Page: | 1218 |
| Language: | English |
| PUBMED: | 8640800 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
