New genes often acquire male-specific functions but rarely become essential in Drosophila Journal Article
| Authors: | Kondo, S.; Vedanayagam, J.; Mohammed, J.; Eizadshenass, S.; Kan, L.; Pang, N.; Aradhya, R.; Siepel, A.; Steinhauer, J.; Lai, E. C. |
| Article Title: | New genes often acquire male-specific functions but rarely become essential in Drosophila |
| Abstract: | Relatively little is known about the in vivo functions of newly emerging genes, especially in metazoans. Although prior RNAi studies reported prevalent lethality among young gene knockdowns, our phylogenomic analyses reveal that young Drosophila genes are frequently restricted to the nonessential male reproductive system. We performed large-scale CRISPR/Cas9 mutagenesis of “conserved, essential” and “young, RNAi-lethal” genes and broadly confirmed the lethality of the former but the viability of the latter. Nevertheless, certain young gene mutants exhibit defective spermatogenesis and/or male sterility. Moreover, we detected widespread signatures of positive selection on young male-biased genes. Thus, young genes have a preferential impact on male reproductive system function. © 2017 Kondo et al. |
| Keywords: | adult; controlled study; nonhuman; animal tissue; gene; gene expression; drosophila; drosophila melanogaster; testis; mutagenesis; lethality; male fertility; spermatogenesis; male genital system; gene evolution; male; priority journal; article; crispr-cas9 system |
| Journal Title: | Genes and Development |
| Volume: | 31 |
| Issue: | 18 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2017-09-15 |
| Start Page: | 1841 |
| End Page: | 1846 |
| Language: | English |
| DOI: | 10.1101/gad.303131.117 |
| PROVIDER: | scopus |
| PMCID: | PMC5695085 |
| PUBMED: | 29051389 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 4 December 2017 -- Source: Scopus |
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4Kan -
10Vedanayagam -
6
Pang
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