Diagnosis of extrapulmonary legionellosis in allogeneic hematopoietic cell transplant recipients by direct 16S ribosomal ribonucleic acid sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Journal Article

   

Authors:	Franco-Garcia, A.; Varughese, T. A.; Lee, Y. J.; Papanicolaou, G.; Rosenblum, M. K.; Hollmann, T. J.; Koehne, G.; Boulad, F.; Babady, N. E.; Tang, Y. W.; Seo, S. K.
Article Title:	Diagnosis of extrapulmonary legionellosis in allogeneic hematopoietic cell transplant recipients by direct 16S ribosomal ribonucleic acid sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Abstract:	Identifying extrapulmonary legionellosis is difficult due to the lack of clinical suspicion and limitations of conventional microbiologic methods. We present a case series of hematopoietic cell transplant recipients with extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal ribonucleic acid gene Sanger sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Keywords:	pneumonia; microbiology; identification; impact; molecular; diagnostics; cell transplant; allogeneic hematopoietic; pneumophila; infectious-diseases; 16s ribosomal ribonucleic acid gene sequencing; extrapulmonary legionellosis; matrix-assisted laser; desorption/ionization time-of-flight mass spectrometry
Journal Title:	Open Forum Infectious Diseases
Volume:	4
Issue:	3
ISSN:	2328-8957
Publisher:	Oxford University Press
Date Published:	2017-07-01
Start Page:	ofx140
Language:	English
ACCESSION:	WOS:000412357400047
DOI:	10.1093/ofid/ofx140
PROVIDER:	wos
PMCID:	PMC5570015
PUBMED:	28852679
Notes:	Article -- Source: Wos

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