The bacterial condensin MukB compacts DNA by sequestering supercoils and stabilizing topologically isolated loops Journal Article
| Authors: | Kumar, R.; Grosbart, M.; Nurse, P.; Bahng, S.; Wyman, C. L.; Marians, K. J. |
| Article Title: | The bacterial condensin MukB compacts DNA by sequestering supercoils and stabilizing topologically isolated loops |
| Abstract: | MukB is a structural maintenance of chromosome-like protein required for DNA condensation. The complete condensin is a large tripartite complex of MukB, the kleisin, MukF, and an accessory protein, MukE. As found previously, MukB DNA condensation is a stepwise process. We have defined these steps topologically. They proceed first via the formation of negative supercoils that are sequestered by the protein followed by hinge– hinge interactions between MukB dimers that stabilize topologically isolated loops in the DNA. MukB itself is sufficient to mediate both of these topological alterations; neither ATP nor MukEF is required. We show that the MukB hinge region binds DNA and that this region of the protein is involved in sequestration of supercoils. Cells carrying mutations in the MukB hinge that reduce DNA condensation in vitro exhibit nucleoid decondensation in vivo. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. |
| Keywords: | controlled study; unclassified drug; gene mutation; genetics; mutation; nonhuman; proteins; nonhistone protein; metabolism; chromosomal proteins, non-histone; protein dna binding; in vivo study; in vitro study; chemistry; dna; protein multimerization; escherichia coli; in-vivo; molecular interaction; adenosine triphosphate; in-vitro; condensin; structural maintenance; bacterial dna; repressor protein; repressor proteins; dna, bacterial; escherichia coli proteins; condensation; topology; escherichia coli protein; dna supercoiling; dna, superhelical; dimer; priority journal; article; decondensation; dna condensation; nucleoids; protein mukb; protein mukef; mukb protein, e coli; muke protein, e coli; mukf protein, e coli; hinge region |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 292 |
| Issue: | 41 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2017-10-01 |
| Start Page: | 16904 |
| End Page: | 16920 |
| Language: | English |
| DOI: | 10.1074/jbc.M117.803312 |
| PUBMED: | 28842486 |
| PROVIDER: | scopus |
| PMCID: | PMC5641887 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 November 2017 -- Source: Scopus |
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