The MukB–topoisomerase IV interaction is required for proper chromosome compaction Journal Article
| Authors: | Kumar, R.; Nurse, P.; Bahng, S.; Lee, C. M.; Marians, K. J. |
| Article Title: | The MukB–topoisomerase IV interaction is required for proper chromosome compaction |
| Abstract: | The bacterial condensin MukB and the cellular decatenating enzyme topoisomerase IV interact. This interaction stimulates intramolecular reactions catalyzed by topoisomerase IV, supercoiled DNA relaxation, and DNA knotting but not intermolecular reactions such as decatenation of linked DNAs. We have demonstrated previously that MukB condenses DNA by sequestering negative supercoils and stabilizing topologically isolated loops in the DNA. We show here that the MukB–topoisomerase IV interaction stabilizes MukB on DNA, increasing the extent of DNA condensation without increasing the amount of MukB bound to the DNA. This effect does not require the catalytic activity of topoisomerase IV. Cells carrying a mukB mutant allele that encodes a protein that does not interact with topoisomerase IV exhibit severe nucleoid decompaction leading to chromosome segregation defects. These findings suggest that the MukB-topoisomerase IV complex may provide a scaffold for DNA condensation. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. |
| Keywords: | controlled study; unclassified drug; genetics; mutation; nonhuman; nonhistone protein; metabolism; chromosomal proteins, non-histone; complex formation; protein dna binding; protein protein interaction; protein stability; in vivo study; wild type; chemistry; dna; escherichia coli; nucleic acids; multiprotein complexes; catalysis; condensin; bacterial dna; dna, bacterial; chromosomes; mutant; dna topoisomerase iv; protein parc; chromosome segregation; chromosomes, bacterial; escherichia coli proteins; catalyst activity; condensation; multiprotein complex; escherichia coli protein; dna supercoiling; dna, superhelical; bacterial chromosome; supercoiled dna; priority journal; article; mutant alleles; intermolecular reactions; intramolecular reactions; topoisomerase iv; dna condensation; protein mukb; mukb protein, e coli; dna knotting |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 292 |
| Issue: | 41 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2017-10-13 |
| Start Page: | 16921 |
| End Page: | 16932 |
| Language: | English |
| DOI: | 10.1074/jbc.M117.803346 |
| PUBMED: | 28842485 |
| PROVIDER: | scopus |
| PMCID: | PMC5641886 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 November 2017 -- Source: Scopus |
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