MukB-mediated catenation of DNA is ATP and MukEF independent Journal Article
| Authors: | Bahng, S.; Hayama, R.; Marians, K. J. |
| Article Title: | MukB-mediated catenation of DNA is ATP and MukEF independent |
| Abstract: | Properly condensed chromosomes are necessary for accurate segregation of the sisters after DNA replication. The Escherichia coli condesin is MukB, a structural maintenance of chromosomes (SMC)-like protein, which forms a complex with MukE and the kleisin MukF. MukB is known to be able to mediate knotting of a DNA ring, an intramolecular reaction. In our investigations of how MukB condenses DNA we discovered that it can also mediate catenation of two DNA rings, an intermolecular reaction. This activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc. |
| Keywords: | proteins; dna; escherichia coli; dna replications; dna binding; structural maintenance; chromosomes; distal regions; intermolecular reactions; intramolecular reactions |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 291 |
| Issue: | 46 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2016-11-11 |
| Start Page: | 23999 |
| End Page: | 24008 |
| Language: | English |
| DOI: | 10.1074/jbc.M116.749994 |
| PROVIDER: | scopus |
| PMCID: | PMC5104925 |
| PUBMED: | 27697840 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 December 2016 -- Source: Scopus |
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