Sequencing Spo11 oligonucleotides for mapping meiotic DNA double-strand breaks in yeast Journal Article

  


Authors: Lam, I.; Mohibullah, N.; Keeney, S.
Article Title: Sequencing Spo11 oligonucleotides for mapping meiotic DNA double-strand breaks in yeast
Abstract: Meiosis is a specialized form of cell division resulting in reproductive cells with a reduced, usually haploid, genome complement. A key step after premeiotic DNA replication is the occurrence of homologous recombination at multiple places throughout the genome, initiated with the formation of DNA double-strand breaks (DSBs) catalyzed by the topoisomerase-like protein Spo11. DSBs are distributed non-randomly in genomes, and understanding the mechanisms that shape this distribution is important for understanding how meiotic recombination influences heredity and genome evolution. Several methods exist for mapping where Spo11 acts. Of these, sequencing of Spo11-associated oligonucleotides (Spo11 oligos) is the most precise, specifying the locations of DNA breaks to the base pair. In this chapter we detail the steps involved in Spo11-oligo mapping in the SK1 strain of budding yeast Saccharomyces cerevisiae, from harvesting cells of highly synchronous meiotic cultures, through preparation of sequencing libraries, to the mapping pipeline used for processing the data. © Springer Science+Business Media New York 2017.
Keywords: meiosis; immunoprecipitation; yeast; dna double-strand breaks; recombination; spo11; genome-wide mapping; spo11 oligos
Journal Title: Methods in Molecular Biology
Volume: 1471
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2017-01-01
Start Page: 51
End Page: 98
Language: English
DOI: 10.1007/978-1-4939-6340-9_3
PROVIDER: scopus
PUBMED: 28349390
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016987942&doi=10.1007%2f978-1-4939-6340-9_3&partnerID=40&md5=ecb3da24acf39705ab3ea33671c42657
Notes: Chapter 3 in "Meiosis. 2nd ed" (ISBN: 978-1-4939-6338-6) -- Export Date: 2 May 2017 -- Source: Scopus
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MSK Authors
  1. Neeman Mohibullah
    15 Mohibullah
  2. Scott N Keeney
    138 Keeney
  3. Isabel Lam
    8 Lam
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