A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation Journal Article

   

Authors:	Pan, J.; Sasaki, M.; Kniewel, R.; Murakami, H.; Blitzblau, H. G.; Tischfield, S. E.; Zhu, X.; Neale, M. J.; Jasin, M.; Socci, N. D.; Hochwagen, A.; Keeney, S.
Article Title:	A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation
Abstract:	The nonrandom distribution of meiotic recombination influences patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in the accumulation of Spo11 protein covalently bound to small DNA fragments. By sequencing these fragments, we uncover a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore how DSB distribution is influenced by large-scale chromosome structures, chromatin, transcription factors, and local sequence composition. Our analysis offers mechanistic insight into DSB formation and early processing steps, supporting the view that the recombination terrain is molded by combinatorial and hierarchical interaction of factors that work on widely different size scales. This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We also discuss implications for evolutionary dynamics of recombination hot spots. © 2011 Elsevier Inc.
Keywords:	sensitivity analysis; chromosome structure; telomere; meiosis; genome-wide association study; transcription factor; evolution; correlation analysis; saccharomyces cerevisiae; chromatin; recombination, genetic; quantitative analysis; nucleotide sequence; dna breaks, double-stranded; chromosome rearrangement; double stranded dna break; binding site; genome; yeast; saccharomyces cerevisiae proteins; spo11 protein; nucleotide; endodeoxyribonucleases; nucleosome; genome, fungal; inheritance
Journal Title:	Cell
Volume:	144
Issue:	5
ISSN:	0092-8674
Publisher:	Cell Press
Date Published:	2011-03-04
Start Page:	719
End Page:	731
Language:	English
DOI:	10.1016/j.cell.2011.02.009
PUBMED:	21376234
PROVIDER:	scopus
PMCID:	PMC3063416
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-79952295560&partnerID=40&md5=5c5b1c1e5ba89278f2a083e09375bbfd
Notes:	--- - "Cited By (since 1996): 1" - "Export Date: 23 June 2011" - "CODEN: CELLB" - "Source: Scopus"

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