Evaluation of breast tissue with confocal strip-mosaicking microscopy: A test approach emulating pathology-like examination Journal Article
| Authors: | Abeytunge, S.; Larson, B.; Peterson, G.; Morrow, M.; Rajadhyaksha, M.; Murray, M. P. |
| Article Title: | Evaluation of breast tissue with confocal strip-mosaicking microscopy: A test approach emulating pathology-like examination |
| Abstract: | Confocal microscopy is an emerging technology for rapid imaging of freshly excised tissue without the need for frozen- or fixed-section processing. Initial studies have described imaging of breast tissue using fluorescence confocal microscopy with small regions of interest, typically 750×750 μm2. We present exploration with a microscope, termed confocal strip-mosaicking microscope (CSM microscope), which images an area of 2×2 cm2 of tissue with cellular-level resolution in 10 min of excision. Using the CSM microscope, we imaged 34 fresh, human, large breast tissue specimens from 18 patients, blindly analyzed by a board-certified pathologist and subsequently correlated with the corresponding standard fixed histopathology. Invasive tumors and benign tissue were clearly identified in CSM strip-mosaic images. Thirty specimens were concordant for image-to-histopathology correlation while four were discordant. © The Authors. The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication,including its DOI. |
| Keywords: | microscopes; confocal microscopy; pathology; medical imaging; tissue engineering; tissue; breast pathology; emerging technologies; fluorescence confocal microscopy; mosaicking; strip mosaicking; breast tissues; cellular-level resolution; invasive tumors |
| Journal Title: | Journal of Biomedical Optics |
| Volume: | 22 |
| Issue: | 3 |
| ISSN: | 1083-3668 |
| Publisher: | SPIE |
| Date Published: | 2017-03-01 |
| Start Page: | 034002 |
| Language: | English |
| DOI: | 10.1117/1.jbo.22.3.034002 |
| PROVIDER: | scopus |
| PMCID: | PMC5361391 |
| PUBMED: | 28327961 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 May 2017 -- Source: Scopus |
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