Dimorphic primers derived from intron 1 for use in the molecular typing of HLA-B alleles Journal Article
| Authors: | Cereb, N.; Yang, S. Y. |
| Article Title: | Dimorphic primers derived from intron 1 for use in the molecular typing of HLA-B alleles |
| Abstract: | We have identified a dimorphic site in intron 1 of the HLA-B gene. Oligotyping was performed on about 3000 samples using primers derived from this dimorphic site in combination with a locus-specific primer derived from intron 3. The distribution of B-alleles bearing each of the dimorphic sequences was approximately equal. These primers were mutually exclusive and yielded approximately 50% of the heterozygous samples as apparently homozygous in PCR products. Intermediate and almost high-resolution oligotyping of HLA-B alleles was achieved using 35 and 63 hybridization probes, respectively. This dimorphic site will provide a useful tool for other PCR-based HLA-B typing approaches. |
| Keywords: | controlled study; human cell; molecular genetics; polymerase chain reaction; allele; intron; alleles; introns; dna primers; hla b antigen; hla typing; hla-b antigens; polymorphism; hybridization; histocompatibility testing; immunogenetics; humans; human; priority journal; article; hla class i; dna typing; intronic sequence |
| Journal Title: | Tissue Antigens |
| Volume: | 50 |
| Issue: | 1 |
| ISSN: | 0001-2815 |
| Publisher: | Wiley-Blackwell Publishing, Inc. |
| Date Published: | 1997-07-01 |
| Start Page: | 74 |
| End Page: | 76 |
| Language: | English |
| PUBMED: | 9243761 |
| PROVIDER: | scopus |
| DOI: | 10.1111/j.1399-0039.1997.tb02839.x |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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