Error rate for HLA-B antigen assignment by serology: Implications for proficiency testing and utilization of DNA-based typing methods Journal Article

  

Authors:	Bozón, M. V.; Delgado, J. C.; Selvakumar, A.; Clavijo, O. P.; Salazar, M.; Ohashi, M.; Alosco, S. M.; Russell, J.; Yu, N.; Dupont, B.; Yunis, E. J.
Article Title:	Error rate for HLA-B antigen assignment by serology: Implications for proficiency testing and utilization of DNA-based typing methods
Abstract:	Until recently, the majority of HLA class I typing has been performed by serology. Expensive commercial typing trays are frequently used for testing non-Caucasian subjects and new strategies using DNA-based methods have been adopted for improving clinical histocompatibility testing results and adapted as supplements in proficiency testing. A double-blind comparison of the typing of HLA-B specificities in 40 samples was carried out between serology and two polymerase chain reaction (PCR) methods, PCR amplification with sequence-specific primers (PCR-SSP) and PCR amplification and subsequent hybridization with sequence-specific oligonucleotide probes (PCR-SSOP). The results demonstrated 22.5% misassignments of HLA-B antigens by serology. There was complete concordance between the results obtained with the two PCR based typing methods. A second panel of 20 donor samples with incomplete or ambiguous serologic results was analyzed by PCR-SSP and SSOP. Both PCR methods identified correctly the HLA-B antigens. Our results suggest that more accurate typing results can be achieved by complementing serologic testing with DNA-based typing techniques. The level of resolution for HLA-B antigen assignment can be obtained by this combination of serology and limited DNA-based typing is equivalent to the HLA-B specificities defined by the WHO-HLA Committee. This level of resolution cannot routinely be achieved in clinical histocompatibility testing or in proficiency testing using serologic reagents only.
Keywords:	sensitivity and specificity; polymerase chain reaction; genotype; dna; diagnostic errors; intermethod comparison; dna primers; evaluation studies; hla b antigen; hla typing; serology; hla-b antigens; normal human; continental population groups; sequence analysis, dna; histocompatibility testing; histocompatibility test; genes, mhc class i; oligonucleotide probe; reagent kits, diagnostic; serologic tests; humans; human; priority journal; article; proficiency testing; sequence-specific oligonucleotide probes; dna probes, hla
Journal Title:	Tissue Antigens
Volume:	50
Issue:	4
ISSN:	0001-2815
Publisher:	Wiley-Blackwell Publishing, Inc.
Date Published:	1997-10-01
Start Page:	387
End Page:	394
Language:	English
PUBMED:	9349624
PROVIDER:	scopus
DOI:	10.1111/j.1399-0039.1997.tb02892.x
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-9844237574&partnerID=40&md5=c94418a584912a339db8a9f4e51e5d50
Notes:	Article -- Export Date: 17 March 2017 -- Source: Scopus

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