Allele assignment for HLA-A, -B, and -C genes to the Tenth International Histocompatibility Workshop cell lines Journal Article
| Authors: | Prasad, V. K.; Yang, S. Y. |
| Article Title: | Allele assignment for HLA-A, -B, and -C genes to the Tenth International Histocompatibility Workshop cell lines |
| Abstract: | Development of DNA typing for Class I HLA alleles has lagged behind that of class II for a variety of technical reasons. Following the recognition of locus specific sequences in the first and the third intron, and acquiring the ability to amplify genomic DNA by intron-based PCR primer, we have devised DNA typing of class I alleles by SSOP and direct sequencing. In this study using these techniques we provide the allelic typing of HLA-A, -B, and -C genes for the B-lymphoblastoid reference cell lines from the Tenth International Histocompatibility Workshop. We also describe some common associations of the C alleles with HLA-A and HLA-B alleles. |
| Keywords: | gene sequence; human cell; polymerase chain reaction; allele; gene amplification; gene locus; alleles; education; hla antigen class 1; hla-c antigens; cell line, transformed; dna sequence; hla a antigen; hla b antigen; hla c antigen; hla typing; dna determination; hla-a antigens; hla-b antigens; histocompatibility; oligonucleotide probe; humans; human; priority journal; article; dna typing; histocompatibility gene; pcr-ssop; workshop cell line |
| Journal Title: | Tissue Antigens |
| Volume: | 47 |
| Issue: | 6 |
| ISSN: | 0001-2815 |
| Publisher: | Wiley-Blackwell Publishing, Inc. |
| Date Published: | 1996-06-01 |
| Start Page: | 538 |
| End Page: | 546 |
| Language: | English |
| PUBMED: | 8813743 |
| PROVIDER: | scopus |
| DOI: | 10.1111/j.1399-0039.1996.tb02596.x |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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