Effects of garlic thioallyl derivatives on growth, glutathione concentration, and polyamine formation of human prostate carcinoma cells in culture Journal Article


Authors: Pinto, J. T.; Qiao, C.; Xing, J.; Rivlin, R. S.; Protomastro, M. L.; Weissler, M. L.; Tao, Y.; Thaler, H.; Heston, W. D. W.
Article Title: Effects of garlic thioallyl derivatives on growth, glutathione concentration, and polyamine formation of human prostate carcinoma cells in culture
Abstract: This study investigated whether naturally occurring garlic derivatives and synthetic S-cysteinyl compounds that resemble garlic constituents have antiproliferative effects on human prostate carcinoma (LNCaP) cells. Studies also examined whether S-allylmercaptocysteine and S-allylcysteine affect two important molecular targets, namely reduced glutathione and polyamines. Results showed that S-allylmercaptocysteine (50 mg/L) diminished LNCaP cell growth whereas the antiproliferative effect of S-allylcysteine was not as pronounced. Studies using synthetic S-cysteinyl analogues revealed that growth inhibition was most effective with compounds containing a disulfide or an active diallyl moiety. Marginal to no inhibitory effect was observed with monosulfinic analogues. Both S-allylmercaptocysteine and S-allylcysteine caused an increase in LNCaP cell reduced glutathione concentrations. Putrescine and spermine concentrations decreased and spermidine increased 3 d after S-allylmercaptecysteine treatment. At 5 d after S- allylmercaptocysteine treatment, polyamine concentrations were similar to those of saline-treated controls. Diminished cell growth and altered polyamine concentrations suggest that S-allylmercaptocysteine may impede the polyamine synthesizing enzyme, ornithine decarboxylase, either by enhancing the formation of reduced glutathione, a known inhibitor of omithine decarboxylase, or by reacting directly with ornithine decarboxylase at its nucleophilic thiol moiety. Because S-allylcysteine also increases reduced glutathione formation but does not significantly inhibit growth, the latter mechanism may be more likely for this compound. These data provide further evidence that nonessential nutrients derived from garlic may modulate tumor growth. Further research is required on effects of garlic derivatives in vivo before information from the present studies can be used to assist in the development of effective nutritional strategies for preventing progression of prostate cancer.
Keywords: controlled study; unclassified drug; human cell; cell division; antineoplastic agents, phytogenic; antineoplastic activity; cancer cell culture; drug effect; drug screening; tumor cells, cultured; prostate cancer; prostatic neoplasms; plant extracts; allium sativum; glutathione; cysteine; plants, medicinal; garlic; polyamine; cysteine derivative; polyamines; garlic extract; putrescine; spermidine; spermine; humans; human; male; article; s allylcysteine; s allylmercaptocysteine; garlic constituents; reduced glutathione; s-allylcysteine; s-allylmercaptocysteine; s-cysteinyl analogues; polyamine synthesis
Journal Title: American Journal of Clinical Nutrition
Volume: 66
Issue: 2
ISSN: 0002-9165
Publisher: American Society for Nutrition  
Date Published: 1997-08-01
Start Page: 398
End Page: 405
Language: English
PUBMED: 9250120
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 17 March 2017 -- Source: Scopus