Reversal of the low-affinity neurotrophin receptor stromal-epithelial expression pattern between benign and malignant human prostate Journal Article

Authors: Papandreou, C. N.; Bogenrieder, T.; Finstad, C. L.; Freeman, R. H.; Chao, M. V.; Albino, A. P.; Scher, H. I.; Reuter, V. E.; Nanus, D. M.
Article Title: Reversal of the low-affinity neurotrophin receptor stromal-epithelial expression pattern between benign and malignant human prostate
Abstract: Reduced expression of the low-affinity p75 neurotrophin receptor (p75(NTR)) occurs in prostate epithelial cells during malignant transformation. Recent studies indicating that the p75(NTR) can transduce signals that induce apoptosis suggest that diminished p75(NTR) in transformed prostate cells may contribute to immortalization. Mutations in the transmembrane domain of the p75(NTR) gene have been associated with decreased p75(NTR) protein expression and may block the ability of the p75(NTR) to induce apoptosis. Therefore, we used Western blot to analyze prostate cancer (PC) cell lines for p75(NTR) protein expression and gene single strand conformation polymorphism (SSCP) analysis and direct DNA sequencing to analyze mutations in the transmembrane domain of the p75(NTR). p75(NTR) Protein was present in all cell lines, and mutations in the p75(NTR) gene were not detected in cDNA derived from any cell line. To define the expression pattern of p75(NTR) in PCs in vivo, we used immunohistochemical techniques to examine tissue specimens from 20 benign, 19 malignant primary, and 14 metastatic prostate specimens. In benign prostate tissues, expression of p75(NTR) was universally detected in basal cells but not in secretory epithelial of stromal cells. In both primary and metastatic PC tissues, p75(NTR) immunoreactivity could not be detected in malignant prostate epithelial cells. However, in contrast to the benign prostate, p75(NTR) protein was expressed in stromal cells surrounding malignant epithelial cells. Stromal p75(NTR) expression was present in 84% (16 of 19) primary and in 86% (12 of 14) metastatic specimens. These data show that in the benign prostate p75(NTR) protein is expressed by basal cells and not stromal cells whereas in malignant prostate p75(NTR) protein is expressed by stromal cells but not prostatic carcinoma cells. Reversal of the p75(NTR) stromal- epithelial pattern of expression between benign and malignant prostate suggests that p75(NTR) may contribute to the development and maintenance of prostate cancer.
Keywords: immunohistochemistry; protein expression; human cell; apoptosis; prostate cancer; stroma; receptor affinity; malignant transformation; single strand conformation polymorphism; neurotrophin receptor; human; male; priority journal; article; neurotrophin receptors
Journal Title: Urologic Oncology: Seminars and Original Investigations
Volume: 4
Issue: 6
ISSN: 1078-1439
Publisher: Elsevier Inc.  
Date Published: 1998-11-01
Start Page: 210
End Page: 217
Language: English
DOI: 10.1016/s1078-1439(98)00036-2
PROVIDER: scopus
PUBMED: 21227260
Notes: Source: Scopus
Altmetric Score
MSK Authors
  1. Victor Reuter
    999 Reuter
  2. Howard Scher
    903 Scher
  3. David M. Nanus
    47 Nanus
  4. Connie L. Finstad
    28 Finstad