Mutation and expression of the low affinity neurotrophin receptor in human malignant melanoma Journal Article

  


Authors: Papandreou, C.; Bogenrieder, T.; Loganzo, F.; Chao, M. V.; Nanus, D. M.; Albino, A. P.
Article Title: Mutation and expression of the low affinity neurotrophin receptor in human malignant melanoma
Abstract: The low affinity p75 neurotrophin receptor (p75(NTR)) is a cysteine-rich transmembrane glycoprotein which is frequently overexpressed in advanced stages of human melanoma. The biological consequences of this overexpression are unknown; however, it has recently been shown that p75(NTR) can enhance the invasive potential of melanoma cells in vitro. In the present study we examined cell lines established from normal human melanocytes and metastatic melanomas for expression of p75(NTR) mRNA and protein. The results showed that, compared with normal melanocytes, levels of p75(NTR)-specific protein were high in seven melanoma lines, markedly decreased in two melanoma lines and comparable in two melanoma lines. The conserved transmembrane domain of p75(NTR) was analysed for point mutations by single strand conformation polymorphism analysis and direct DNA sequencing. Identical point mutations were detected in the transmembrane domain of p75(NTR) in the two melanoma lines with reduced p75(NTR) protein expression, which resulted in the substitution of the uncharged amino acid Gly for the negatively-charged Asp.
Keywords: controlled study; human cell; mutation; polymerase chain reaction; cytology; cells, cultured; melanoma; melanocyte; melanocytes; tumor cells, cultured; blotting, western; base sequence; point mutation; sequence analysis, dna; receptor, nerve growth factor; receptors, nerve growth factor; polymorphism, single-stranded conformational; neurotrophin; neurotrophin receptor; humans; human; priority journal; article; p75 receptor
Journal Title: Melanoma Research
Volume: 6
Issue: 5
ISSN: 0960-8931
Publisher: Lippincott Williams & Wilkins  
Date Published: 1996-10-01
Start Page: 373
End Page: 378
Language: English
DOI: 10.1097/00008390-199610000-00004
PUBMED: 8908597
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029910296&doi=10.1097%2f00008390-199610000-00004&partnerID=40&md5=a51ffe7cb3579cb572c22bf0305dbd78
Notes: Source: Scopus
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MSK Authors
  1. Christos N Papandreou
    20 Papandreou
  2. David M. Nanus
    66 Nanus
  3. Anthony P. Albino
    111 Albino
  4. Thomas Bogenrieder
    18 Bogenrieder
  5. Frank Loganzo Jr
    15 Loganzo
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