A drosophila pasha mutant distinguishes the canonical microRNA and mirtron pathways Journal Article
| Authors: | Martin, R.; Smibert, P.; Yalcin, A.; Tyler, D. M.; Schafer, U.; Tuschl, T.; Lai, E. C. |
| Article Title: | A drosophila pasha mutant distinguishes the canonical microRNA and mirtron pathways |
| Abstract: | Canonical primary microRNA (miRNA) transcripts and mirtrons are proposed to transit distinct nuclear pathways en route to generating mature ~22 nucleotide regulatory RNAs. We generated a null allele of Drosophila pasha, which encodes a double-stranded RNA-binding protein partner of the RNase III enzyme Drosha. Analysis of this mutant yielded stringent evidence that Pasha is essential for the biogenesis of canonical miRNAs but is dispensable for the processing and function of mirtron-derived regulatory RNAs. The pasha mutant also provided a unique tool to study the developmental requirements for Drosophila miRNAs. While pasha adult somatic clones are similar in many respects to those of dicer-1 clones, pasha mutant larvae revealed an unexpected requirement for the miRNA pathway in imaginal disc growth. These data suggest limitations to somatic clonal analysis of miRNA pathway components. Copyright © 2009, American Society for Microbiology. All Rights Reserved. |
| Keywords: | controlled study; unclassified drug; gene deletion; genetics; mutation; nonhuman; phenotype; animal; metabolism; animals; allele; somatic cell; microrna; gene expression; biological model; models, biological; intron; alleles; introns; drosophila; pathology; rna binding protein; growth, development and aging; rna-binding proteins; homozygote; drosophila melanogaster; cell clone; micrornas; rna processing; drosophila proteins; clone cells; regulatory rna sequence; biogenesis; drosophila protein; dicer; dicer 1; double stranded rna; drosophila protein pasha; ribonuclease iii; pasha protein, drosophila; embryo growth; imaginal disc; null allele; eye; larva; rna processing, post-transcriptional |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 29 |
| Issue: | 3 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2009-02-01 |
| Start Page: | 861 |
| End Page: | 870 |
| Language: | English |
| DOI: | 10.1128/mcb.01524-08 |
| PUBMED: | 19047376 |
| PROVIDER: | scopus |
| PMCID: | PMC2630677 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 5" - "Export Date: 30 November 2010" - "CODEN: MCEBD" - "Source: Scopus" |
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