ATmprss2-CreER(T2) knock-in mouse model for cancer genetic studies on prostate and colon Journal Article


Authors: Gao, D.; Zhan, Y.; Di, W.; Moore, A. R.; Sher, J. J.; Guan, Y.; Wang, S.; Zhang, Z.; Murphy, D. A.; Sawyers, C. L.; Chi, P.; Chen, Y.
Article Title: ATmprss2-CreER(T2) knock-in mouse model for cancer genetic studies on prostate and colon
Abstract: Fusion between TMPRSS2 and ERG, placing ERG under the control of the TMPRSS2 promoter, is the most frequent genetic alteration in prostate cancer, present in 40-50% of cases. The fusion event is an early, if not initiating, event in prostate cancer, implicating the TMPRSS2-positive prostate epithelial cell as the cancer cell of origin in fusion-positive prostate cancer. To introduce genetic alterations into Tmprss2-positive cells in mice in a temporal- specific manner, we generated a Tmprss2-CreERT2 knock-in mouse. We found robust tamoxifen-dependent Cre activation in the prostate luminal cells but not basal epithelial cells, as well as epithelial cells of the bladder and gastrointestinal (GI) tract. The knock-in allele on the Tmprss2 locus does not noticeably impact prostate, bladder, or gastrointestinal function. Deletion of Pten in Tmprss2-positive cells of adult mice generated neoplasia only in the prostate, while deletion of Apc in these cells generated neoplasia only in the GI tract. These results suggest that this new Tmprss2-CreERT2 mouse model will be a useful resource for genetic studies on prostate and colon. © 2016 Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Journal Title: PLoS ONE
Volume: 11
Issue: 8
ISSN: 1932-6203
Publisher: Public Library of Science  
Date Published: 2016-08-18
Start Page: e0161084
Language: English
DOI: 10.1371/journal.pone.0161084
PROVIDER: scopus
PMCID: PMC4990297
PUBMED: 27536883
DOI/URL:
Notes: Article -- Export Date: 3 October 2016 -- Source: Scopus
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MSK Authors
  1. Charles L Sawyers
    226 Sawyers
  2. Yu Chen
    133 Chen
  3. Ping Chi
    173 Chi
  4. Dong Gao
    28 Gao
  5. Devan Anne Murphy
    11 Murphy
  6. Zeda Zhang
    18 Zhang
  7. Amanda Roxanne Moore
    10 Moore
  8. Jessica   Sher
    13 Sher
  9. Youxin   Guan
    11 Guan
  10. Yu   Zhan
    6 Zhan
  11. Shangqian   Wang
    20 Wang