Biophysical differences between chronic myelogenous leukemic quiescent and proliferating stem/progenitor cells Journal Article

   

Authors:	Guz, N. V.; Patel, S. J.; Dokukin, M. E.; Clarkson, B.; Sokolov, I.
Article Title:	Biophysical differences between chronic myelogenous leukemic quiescent and proliferating stem/progenitor cells
Abstract:	The treatment of chronic myeloid leukemia (CML), a clonal myeloproliferative disorder has improved recently, but most patients have not yet been cured. Some patients develop resistance to the available tyrosine kinase treatments. Persistence of residual quiescent CML stem cells (LSCs) that later resume proliferation is another common cause of recurrence or relapse of CML. Eradication of quiescent LSCs is a promising approach to prevent recurrence of CML. Here we report on new biophysical differences between quiescent and proliferating CD34+ LSCs, and speculate how this information could be of use to eradicate quiescent LSCs. Using AFM measurements on cells collected from four untreated CML patients, substantial differences are observed between quiescent and proliferating cells in the elastic modulus, pericellular brush length and its grafting density at the single cell level. The higher pericellular brush densities of quiescent LSCs are common for all samples. The significance of these observations is discussed. © 2016 Elsevier Inc.
Keywords:	myeloproliferative disorders; cell proliferation; cytology; chronic myeloid leukemia; stem cells; amino acids; patient treatment; diseases; personalized medicine; flowcharting; cells; single cell analysis; atomic force microscopy; proliferating cells; singlecell analysis; cell mechanics; chronic myeloid leukemias; grafting densities; personalized medicines; single-cell level
Journal Title:	Nanomedicine-Nanotechnology Biology and Medicine
Volume:	12
Issue:	8
ISSN:	1549-9634
Publisher:	Elsevier Science, Inc.
Date Published:	2016-11-01
Start Page:	2429
End Page:	2437
Language:	English
DOI:	10.1016/j.nano.2016.06.016
PROVIDER:	scopus
PUBMED:	27431055
PMCID:	PMC5116407
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984636149&partnerID=40&md5=14c24900abc58dd9fa3b4f7a97bea9ca
Notes:	Article -- Export Date: 3 October 2016 -- Source: Scopus

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