Gene expression differences between enriched normal and chronic myelogenous leukemia quiescent stem/progenitor cells and correlations with biological abnormalities Journal Article


Authors: Affer, M.; Su, D.; Liu, C.; Olshen, A. B.; Mo, Q.; Viale, A.; Lambek, C. L.; Marr, T. G.; Clarkson, B. D
Article Title: Gene expression differences between enriched normal and chronic myelogenous leukemia quiescent stem/progenitor cells and correlations with biological abnormalities
Abstract: In comparing gene expression of normal and CML CD34+ quiescent (G0) cell, 292 genes were downregulated and 192 genes upregulated in the CML/G0 Cells. The differentially expressed genes were grouped according to their reported functions, and correlations were sought with biological differences previously observed between the same groups. The most relevant findings include the following. (i) CML G0 cells are in a more advanced stage of development and more poised to proliferate than normal G0 cells. (ii) When CML G0 cells are stimulated to proliferate, they differentiate and mature more rapidly than normal counterpart. (iii) Whereas normal G0 cells form only granulocyte/monocyte colonies when stimulated by cytokines, CML G0 cells form a combination of the above and erythroid clusters and colonies. (iv) Prominin-1 is the gene most downregulated in CML G0 cells, and this appears to be associated with the spontaneous formation of erythroid colonies by CML progenitors without EPO. Copyright © 2011 M. Affer et al.
Keywords: clinical article; controlled study; human tissue; human cell; cell proliferation; cd34 antigen; gene expression; cell differentiation; chronic myeloid leukemia; stem cell; gene expression regulation; cytokine; correlation analysis; cell culture; erythroid cell; upregulation; monocyte; cell cycle g0 phase; granulocyte; receptor down regulation
Journal Title: Journal of Oncology
Volume: 2011
ISSN: 1687-8450
Publisher: Hindawi Publishing Corporation  
Date Published: 2011-01-01
Start Page: 798592
Language: English
DOI: 10.1155/2011/798592
PROVIDER: scopus
PMCID: PMC3062978
PUBMED: 21436996
DOI/URL:
Notes: --- - "Export Date: 23 June 2011" - "Art. No.: 798592" - "Source: Scopus"
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MSK Authors
  1. Qianxing Mo
    37 Mo
  2. Dao Su
    8 Su
  3. Agnes Viale
    245 Viale
  4. Chong-Yuan Liu
    11 Liu
  5. Bayard Clarkson
    220 Clarkson
  6. Caryl Lambek
    21 Lambek