Gene expression differences between enriched normal and chronic myelogenous leukemia quiescent stem/progenitor cells and correlations with biological abnormalities Journal Article
| Authors: | Affer, M.; Su, D.; Liu, C.; Olshen, A. B.; Mo, Q.; Viale, A.; Lambek, C. L.; Marr, T. G.; Clarkson, B. D |
| Article Title: | Gene expression differences between enriched normal and chronic myelogenous leukemia quiescent stem/progenitor cells and correlations with biological abnormalities |
| Abstract: | In comparing gene expression of normal and CML CD34+ quiescent (G0) cell, 292 genes were downregulated and 192 genes upregulated in the CML/G0 Cells. The differentially expressed genes were grouped according to their reported functions, and correlations were sought with biological differences previously observed between the same groups. The most relevant findings include the following. (i) CML G0 cells are in a more advanced stage of development and more poised to proliferate than normal G0 cells. (ii) When CML G0 cells are stimulated to proliferate, they differentiate and mature more rapidly than normal counterpart. (iii) Whereas normal G0 cells form only granulocyte/monocyte colonies when stimulated by cytokines, CML G0 cells form a combination of the above and erythroid clusters and colonies. (iv) Prominin-1 is the gene most downregulated in CML G0 cells, and this appears to be associated with the spontaneous formation of erythroid colonies by CML progenitors without EPO. Copyright © 2011 M. Affer et al. |
| Keywords: | clinical article; controlled study; human tissue; human cell; cell proliferation; cd34 antigen; gene expression; cell differentiation; chronic myeloid leukemia; stem cell; gene expression regulation; cytokine; correlation analysis; cell culture; erythroid cell; upregulation; monocyte; cell cycle g0 phase; granulocyte; receptor down regulation |
| Journal Title: | Journal of Oncology |
| Volume: | 2011 |
| ISSN: | 1687-8450 |
| Publisher: | Hindawi Publishing Corporation |
| Date Published: | 2011-01-01 |
| Start Page: | 798592 |
| Language: | English |
| DOI: | 10.1155/2011/798592 |
| PROVIDER: | scopus |
| PMCID: | PMC3062978 |
| PUBMED: | 21436996 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 23 June 2011" - "Art. No.: 798592" - "Source: Scopus" |
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