Langerhans cells: Straight from blood to skin? Journal Article


Authors: Romani, N.; Young, J. W.
Article Title: Langerhans cells: Straight from blood to skin?
Abstract: In this issue of Blood, Milne et al1 report a developmental pathway for human Langerhans cells from circulating CD14-CD1c+ dendritic cell (DC) precursors in peripheral blood. The Langerhans cell progeny, expressing CD207 (langerin) and bearing the hallmark Birbeck granules, result from culture in granulocyte macrophage colony-stimulating factor (GM-CSF) and transforming growth factor-β1 (TGF-β1) and/or bone morphogenetic protein 7 (BMP7). A similar study by Martínez-Cingolani et al,2 also published recently in Blood, identified the same circulating CD14-CD1c+ Langerhans cell precursors. These authors used a different anti-CD1c monoclonal antibody (BDCA-1). In that study, TGF-β1 synergized with thymic stromal lymphopoietin (TSLP) to yield similar Langerhans cell progeny. Both groups used fetal calf serum (FCS), however, which affects differentiation and maturation by unknown mechanisms and is not physiologically comparable to human plasma in vivo. Controls with FCS-containing medium, but without cytokines, indicated that FCS was not the driver of the conversion. However, Milne et al1 could not generate Langerhans cell progeny by culture in X-Vivo medium without FCS, despite inclusion of GM-CSF, TGF-β1, and BMP7, all of which, together with TSLP, are epithelial cell-derived cytokines under physiologic conditions. © 2015 by The American Society of Hematology.
Keywords: protein expression; unclassified drug; nonhuman; note; protein function; phenotype; cytology; metabolism; cell function; cell maturation; dendritic cell; epidermis; granulocyte macrophage colony stimulating factor; culture medium; in vivo study; cell differentiation; in vitro study; monoclonal antibody; skin; cytokine; blood; dendritic cells; cytotoxic t lymphocyte; progeny; transforming growth factor beta1; epithelium cell; blood cell; blood cells; hematopoiesis; hematopoietic stem cell; osteogenic protein 1; monocyte; monocytes; glycoproteins; glycoprotein; plasma; cd14 antigen; monocyte macrophage precursor cell; langerhans cell; langerhans cells; fetal calf serum; cd1 antigen; antigens, cd1; thymic stromal lymphopoietin; humans; human; priority journal; langerin; cd1c antigen; monoclonal antibody bdca 1; cd1c protein, human
Journal Title: Blood
Volume: 125
Issue: 3
ISSN: 0006-4971
Publisher: American Society of Hematology  
Date Published: 2015-01-15
Start Page: 420
End Page: 422
Language: English
DOI: 10.1182/blood-2014-11-610667
PUBMED: 25593330
PROVIDER: scopus
DOI/URL:
Notes: Note -- Export Date: 3 October 2016 -- Source: Scopus
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  1. James W Young
    257 Young