Basic fibroblast growth factor counteracts the suppressive effect of transforming growth factor-β1 on human myeloid progenitor cells Journal Article


Authors: Gabrilove, J. L.; Wong, G.; Bollenbacher, E.; White, K.; Kojima, S.; Wilson, E. L.
Article Title: Basic fibroblast growth factor counteracts the suppressive effect of transforming growth factor-β1 on human myeloid progenitor cells
Abstract: We have previously shown that basic fibroblast growth factor (bFGF) is mitogenic for human bone marrow stromal cells and enhances myelopoiesis in human long-term bone marrow culture. In the present study, we examined the mechanism by which bFGF enhances granulopoiesis. We observed that bFGF significantly abrogated the inhibitory effect of transforming growth factor- β1 (TGF-β1) on granulocyte-macrophage colony-stimulating factor (GM-CSF)- supported progenitor cell growth (P = .009). The partial reversal of TGF- β1-mediated suppression was dependent on the dose of bFGF used. In addition, we noted that the inclusion of neutralizing antibody to TGF-β1 significantly augmented the clonogenic response to GM-CSF. We have also shown that 10 ng/mL or 100 ng/mL of bFGF resulted in a 30% to 100% increase in GM-CSF-mediated progenitor cell growth (P = .0001). These data suggest that bFGF may enhance myelopoiesis by modulating the inhibitory response to TGF-β1.
Keywords: human cell; cell proliferation; cell division; transforming growth factor beta; fibroblast growth factor 2; hematopoietic stem cells; transforming growth factor beta1; hematopoiesis; antibodies; recombinant granulocyte colony stimulating factor; cell stimulation; recombinant granulocyte macrophage colony stimulating factor; colony forming unit gm; clonogenesis; promyelocyte; granulocyte-macrophage colony-stimulating factor; basic fibroblast growth factor; myelopoiesis; immunization, passive; granulocytes; growth stimulation; human; priority journal; article; support, u.s. gov't, p.h.s.
Journal Title: Blood
Volume: 81
Issue: 4
ISSN: 0006-4971
Publisher: American Society of Hematology  
Date Published: 1993-02-15
Start Page: 909
End Page: 915
Language: English
PUBMED: 8427999
PROVIDER: scopus
DOI/URL:
Notes: Source: Scopus